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- W59385587 abstract "The mechanosensory protein 6 (MEC-6) of C. elegans encodes a single-pass membrane protein with sequence homology to the paraoxonase (PON) family of proteins, e.g., 25% identical and 45% similar to PON1 over a stretch of 377 amino acids in its carboxy terminus. The focus of this research was to identify the analogous functions of MEC-6 to the PON family. We examined 1) enzymatic activity of recombinant MEC-6 (rMEC-6), 2) putative anti-oxidative functions of MEC-6 in HeLa cells and 3) role of mec-6 in the protection of C. elegans from oxidative stress. We found rMEC-6 was able to hydrolyze phenyl acetate and dihydrocouramin, two known substrates of PON in a Ca2+-dependent manner, indicating that MEC-6 had PON-like function as an arylesterase and a lactonase. To examine the role of MEC-6 as an anti-oxidant, we created a stable line of HeLa cells expressing MEC-6. Using a redox-sensitive green fluorescent protein (roGFP1) assay, we found that cells overexpressing MEC-6 protein were afforded much better protection from exposure to exogenous oxidants compared to control cells. These data were equivalent to previously demonstrated anti-oxidant capabilities of hPON2. To test the role mec-6 in C. elegans animals, we looked at two different mutant mec-6 alleles and their ability to cope with oxidative insult. Exposing young adult animals to 40 uM H2O2 for 2 hours killed 80% of mec-6 null animals compared to 40% loss in wildtype. Thus, the survival rate was decreased in mec-6 null animals compared to wildtype animals. These studies reveal a role for MEC-6 protein providing protection against oxidative stress. Support by DK10141." @default.
- W59385587 created "2016-06-24" @default.
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- W59385587 date "2006-03-01" @default.
- W59385587 modified "2023-09-24" @default.
- W59385587 title "Anti‐oxidative functions of C. elegans mechanosensory protein 6 (MEC‐6), a homolog for human PON1." @default.
- W59385587 doi "https://doi.org/10.1096/fasebj.20.5.lb22-a" @default.
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