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- W595132324 abstract "Anesthetic-mediated cardioprotection has been shown to decrease myocardial infarct size after ischemia-reperfusion injury. Many cell signaling pathways have been investigated in this phenomenon, however, the role of microRNA remains unknown. MicroRNAs are short, non-coding nucleotide sequences that negatively regulate gene expression through degradation or suppression of messenger RNA. Previously, we have shown that miR-21 is upregulated approximately 2.5-fold after exposure to isoflurane in both in vivo and in vitro rat models. In this study, we investigated the effect of knocking down miR-21 expression on cardioprotection by isoflurane in neonatal rat cardiomyocytes. Knockdown of miR-21 in neonatal rat cardiomyocytes was achieved by transfection with Lipofectamine and confirmed using qRT-PCR. Lactate dehydrogenase release assays were conducted to assess cell viability compared to scramble-transfected controls. Our results show that miR-21 knockdown attenuates the cardioprotective effect of isoflurane in neonatal rat cardiomyocytes. We have also shown a correlation between miR-21 knockdown and decreased expression of pAkt, a well-established mediator of protective cell signaling pathways. Further investigation into the mechanisms by which miR-21 contributes to cardioprotection may offer novel insight into the attenuation of ischemia-reperfusion injury." @default.
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- W595132324 date "2013-04-01" @default.
- W595132324 modified "2023-09-27" @default.
- W595132324 title "miR‐21 Knockdown Attenuates the Cardioprotective Effects of Isoflurane" @default.
- W595132324 doi "https://doi.org/10.1096/fasebj.27.1_supplement.lb679" @default.
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