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• W595759622 abstract "Salmonella newington was cultured with 0-(3)a. (15)a antiserum which was absorbed with the living cells of S. thomasville, and S. newington [E3 (X)], having the antigenic structure of subgroup, was isolated, frozen, thawed and filtered. The directed variation of Salmonella subgroups E1 and E2 was attempted using the fitrate, and antigen transformations from 0-3.15 into 0-(3). (15). 34 were observed. These transformations were always paralleled with the lytic ability of the phage. By pure culture and absorption test of the frozen-thawed filtrate with S. anatum, two kinds of phage were confirmed, each having different ability-one transforms 0-3.10 into 0-3.15 and the other 0-(3). (15) into 0-(3). (15), 34.The two phages were isolated and purified to investigate the relation between phage activity and antigen transforming ability, find it was found that the lytio ability and the antigen transforming ability is inseparable, and that the latter is not affected by the enzymic action of desoxyribonuclease, ribonuclease, trypsin, etc.The phage related to the antigen transformation 0-3.10→0-3.15 is designated ε15, and that related to 0-3.15→0-(3). (15). 34, ε34. Both of them are spherical particles ranging 26-40mμ. in size. ε15 is identical in properties with ε which was previously reported, but ε34 is completely different from it both biochemically and serologically. ε34 is maintained actively for 20 minute at 80°C, but becomes inactive at 80°C 30 minutes and at 85°C in 1.5 minutes. It is active at pH 2.4-12.0 but inactivated below pH 2.2 and above pH 12.5. Its host range is limited to subgroup E2 strains and it can not even be adsorbed by subgroups E1 and E4 strains.The two antigen transforming abilities are inhibited by the respective anti-phage serum together with lystic ability but not affected by the antiserum against different phage.ε34 well transformed strains containing 0-15 into subgroup E3 strains, but could not directly transform subgroups E1 and E4 strains samely as they could not adsorb them. However, when they were infected with ε15 and had 0-15 antigen formed, ε34 can transform them into E3 by infecting them. An transformed variant of S. senftenberg has a double antigenic structure 0-1. (3). (15). (19). 34. It is a very rare strain which has not yet been discovered in nature.The rate of antigen transformation 0-3.15→0-(3). (15). 34 was investigated with S. newington Sr and it was found to be ranging 2.6-10.3%.Strains having antigenic structure of subgroup E3 adsorb ε34, but lysis does not take place. Examination for lysogenic phage revealed that 6 out of 12 tested strains had bath ε15 and ε34, 5 had only ε15, and 1 had neither.A variant of S. newington Sr, having antigenic structure of subgroup E3, was confirmed to be doubly lysogenic bacteria by the quantitative investigation of its lysogenicity" @default.
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• W595759622 date "1956-01-01" @default.
• W595759622 modified "2023-09-25" @default.
• W595759622 title "STUDIES ON DIRECTED VARIATION OF SALMONELLA 0-15 AND 0-34 BY BACTERIOPHAGE [I]" @default.
• W595759622 doi "https://doi.org/10.2222/jsv1951.6.285" @default.
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