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• W59654435 abstract "The leishmaniasis are zoonoses that affect humans and other species of wild and domestic mammals. It is a disease caused by intracellular protozoa of the genus Leishmania. Leishmania infantum (syn. L. chagasi) is the causative agent of visceral leishmaniasis in the New World, Lutzomyia longipalpis is the main vector responsible for transmission. The cat, besides living closely with humans, also develops infection, usually asymptomatic and may also act as a reservoir of these protozoa. Given the specificity of clinical feline leishmaniasis (LF), the absence of pathognomonic signs and symptoms, and the similarity of the clinical aspects of this disease with other very common in cats, as mycoplasmosis, feline leukemia virus (FIV) and immunodeficiency syndrome FeLV (Feline), this zoonosis should be systematically considered in the clinical suspicions of cats in of the areas endemic for canine and human leishmaniasis. Samples of blood from 55 cats coming from the Association for the Protection of Animals (APAISA) of Ilha Solteira, co-inhabitants with dogs with visceral leishmaniasis, were collected. The blood culture technique showed 16,4% (9/55) flagellate protozoa. The technique of indirect fluorescent antibody (IFA) for L. infantum (syn. L. chagasi) was tested on 51 cats, showing 62,7% (32/51) of animals reagents. Testing the technique of IFA for Trypanosoma cruzi, 54,9% (28/55) of animals were reactive. The search for anti-Leishmania antibodies and anti-Trypanosoma, the technique of ELISA with crude antigen, we found 72,5% (37/51) and 39,2% (20/55) of animals reagents, respectively. By ELISA with indirect rK39 for Leishmania infantum (syn. L.chagasi) gave a positive result in 21,6% (11/51) of tested cats. For the PCR, the 55 whole blood samples tested, five (9.1%) were positive for Leishmania spp. Confronting all the results obtained with the diagnostic techniques , is possible to determine the importance of the cats in this study as reservoir of Leishmania spp., and the PCR as an important tool for diagnostic confirmation." @default.
• W59654435 created "2016-06-24" @default.
• W59654435 creator A5068507985 @default.
• W59654435 date "2013-04-19" @default.
• W59654435 modified "2023-09-23" @default.
• W59654435 title "AVALIAÇÃO DIAGNÓSTICA PARA Leishmania spp. E Trypanosoma cruzi EM GATOS DOMÉSTICOS PROCEDENTES DA ASSOCIAÇÃO PROTETORA DOS ANIMAIS DO MUNICÍPIO DE ILHA SOLTEIRA, SP, BRASIL" @default.
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