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- W600199202 abstract "Cotton leaf curl disease (CLCuD) associated with a complex of begomoviruses is an important agricultural issue of cotton. One of the reasons for breakdown of resistance against these viruses is gene silencing (GS) that come out as a problem while developing transgenic resistance in plants. This study is providing a strategy to manage CLCuD by controlling diverse begomoviruses and circumventing GS. A truncated Rep protein (Repsyn130) of a begomovirus optimized for plant expression and avoiding virus-induced gene silencing (VIGS) was designed using bioinformatics tools and commercially synthesized. Nterminus 390 nucleotides encoding 130 amino acids of Reps of nine different begomoviruses involved in CLCuD were aligned with Repsyn130 and confirmed no chances of GS. Resistance evaluation of Repsyn130 against Cotton leaf curl Khokhran virus (CLCuKoV) and Cotton leaf curl Multan betasatellite (CLCuMuB) was performed by Agrobacterium-mediated transient assay in Nicotiana benthamiana plants. Agroinoculation of CLCuKoV/CLCuMuB along with Repsyn130 resulted in 65% resistance by visual observation of symptoms severity along with 5-6 days delayed and milder symptoms development. PCR of 20 experimental plants confirmed the presence of CLCuKoV/CLCuMuB in 90% plants however, Southern analyses showed decreased viral load. These findings showed the potential of the synthetic Rep gene for resistance against CLCuD complex. © 2013 Friends Science Publishers" @default.
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- W600199202 date "2013-01-01" @default.
- W600199202 modified "2023-09-24" @default.
- W600199202 title "Interference of a synthetic rep protein to develop resistance against cotton leaf curl disease." @default.
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