FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W6010908> ?p ?o ?g. }

• W6010908 endingPage "182" @default.
• W6010908 startingPage "175" @default.
• W6010908 abstract "The thyroid-stimulating hormone (TSH) receptor is widely regarded as one of a limited number of G-protein-coupled receptors that activate both adenylate cyclase and phosphoinositidase C (PIC) via G-proteins, but the existing experimental evidence for TSH-stimulated PtdIns(4,5)P2 hydrolysis remains inconclusive. We have compared the effects of TSH and of ATP (acting via P2-purinergic receptors) on the inositol lipids and polyphosphates of [2-3H]inositol-labelled FRTL-5 rat thyroid cells. ATP initiated a rapid decrease in 3H-labelled PtdIns4P and PtdIns(4,5)P2, whereas TSH did not. Stimulation with ATP and, less consistently, with noradrenaline (acting via alpha-adrenergic receptors) provoked rapid formation of Ins(1,4,5)P3, Ins(1,3,4,5)P4, Ins(1,3,4)P3 and Ins(1,4)P2, confirming activation of PtdIns(4,5)P2 hydrolysis. No concentration of TSH provoked detectable accumulation of Ins(1,4,5)P3 or Ins(1,4)P2 during the first few minutes of stimulation. However, an InsP3 [with the chromatographic properties of Ins(1,3,4)P3] and two InsP4 isomers [neither of which was Ins(1,3,4,5)P4] accumulated quickly in TSH-stimulated cells. ATP immediately provoked a large increase in intracellular calcium concentration ([Ca2+]i) in Indo 1-AM-loaded cells. TSH provoked a small and delayed [Ca2+]i elevation in only some experiments. We therefore confirm that activation of P2-purinergic receptors and alpha 1-adrenergic receptors provokes PIC activation, an accumulation of Ins(1,4,5)P3 and its metabolites and rapid [Ca2+]i mobilization in FRTL-5 cells. By contrast, TSH provokes no rapid PIC-catalysed PtdIns(4,5)P2 hydrolysis or immediate [Ca2+]i mobilization. These results fail to support the widespread view that the TSH receptor of FRTL-5 cells signals, in part, through PIC activation. Our results suggest that TSH activates another, still undefined, mechanism that causes accumulation of an InsP3 and two isomers of InsP4." @default.
• W6010908 created "2016-06-24" @default.
• W6010908 creator A5009389483 @default.
• W6010908 creator A5010745038 @default.
• W6010908 creator A5012764646 @default.
• W6010908 creator A5025740932 @default.
• W6010908 creator A5049719396 @default.
• W6010908 creator A5082278561 @default.
• W6010908 date "1996-05-15" @default.
• W6010908 modified "2023-09-30" @default.
• W6010908 title "Thyroid-stimulating hormone rapidly stimulates inositol polyphosphate formation in FRTL-5 thyrocytes without activating phosphoinositidase C" @default.
• W6010908 cites W1509560911 @default.
• W6010908 cites W1551997271 @default.
• W6010908 cites W1594016069 @default.
• W6010908 cites W1608606583 @default.
• W6010908 cites W1617916547 @default.
• W6010908 cites W1772576610 @default.
• W6010908 cites W1795641679 @default.
• W6010908 cites W1815757713 @default.
• W6010908 cites W184070157 @default.
• W6010908 cites W1878565906 @default.
• W6010908 cites W1900249688 @default.
• W6010908 cites W1924889401 @default.
• W6010908 cites W1972113530 @default.
• W6010908 cites W1976457107 @default.
• W6010908 cites W1980166477 @default.
• W6010908 cites W2002003115 @default.
• W6010908 cites W2004579046 @default.
• W6010908 cites W2011674304 @default.
• W6010908 cites W2015150249 @default.
• W6010908 cites W2032332840 @default.
• W6010908 cites W2053149611 @default.
• W6010908 cites W2055290156 @default.
• W6010908 cites W2055698093 @default.
• W6010908 cites W2056591867 @default.
• W6010908 cites W2059080130 @default.
• W6010908 cites W2060992793 @default.
• W6010908 cites W2062848455 @default.
• W6010908 cites W2063865554 @default.
• W6010908 cites W2067321100 @default.
• W6010908 cites W2072099683 @default.
• W6010908 cites W2078833535 @default.
• W6010908 cites W2081186483 @default.
• W6010908 cites W2082395639 @default.
• W6010908 cites W2086348707 @default.
• W6010908 cites W2089816992 @default.
• W6010908 cites W2094911147 @default.
• W6010908 cites W2106516118 @default.
• W6010908 cites W2115907343 @default.
• W6010908 cites W2116383891 @default.
• W6010908 cites W2206429895 @default.
• W6010908 cites W2207533602 @default.
• W6010908 cites W2304467204 @default.
• W6010908 cites W2343892241 @default.
• W6010908 cites W2410670672 @default.
• W6010908 cites W2418053496 @default.
• W6010908 cites W941269249 @default.
• W6010908 doi "https://doi.org/10.1042/bj3160175" @default.
• W6010908 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1217319" @default.
• W6010908 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8645202" @default.
• W6010908 hasPublicationYear "1996" @default.
• W6010908 type Work @default.
• W6010908 sameAs 6010908 @default.
• W6010908 citedByCount "22" @default.
• W6010908 countsByYear W60109082012 @default.
• W6010908 countsByYear W60109082013 @default.
• W6010908 crossrefType "journal-article" @default.
• W6010908 hasAuthorship W6010908A5009389483 @default.
• W6010908 hasAuthorship W6010908A5010745038 @default.
• W6010908 hasAuthorship W6010908A5012764646 @default.
• W6010908 hasAuthorship W6010908A5025740932 @default.
• W6010908 hasAuthorship W6010908A5049719396 @default.
• W6010908 hasAuthorship W6010908A5082278561 @default.
• W6010908 hasBestOaLocation W60109082 @default.
• W6010908 hasConcept C126322002 @default.
• W6010908 hasConcept C134018914 @default.
• W6010908 hasConcept C163235415 @default.
• W6010908 hasConcept C170493617 @default.
• W6010908 hasConcept C180526460 @default.
• W6010908 hasConcept C185592680 @default.
• W6010908 hasConcept C24998067 @default.
• W6010908 hasConcept C2777427919 @default.
• W6010908 hasConcept C2778597717 @default.
• W6010908 hasConcept C2778618036 @default.
• W6010908 hasConcept C2780069284 @default.
• W6010908 hasConcept C55493867 @default.
• W6010908 hasConcept C57306754 @default.
• W6010908 hasConcept C71924100 @default.
• W6010908 hasConcept C80631254 @default.
• W6010908 hasConcept C86803240 @default.
• W6010908 hasConceptScore W6010908C126322002 @default.
• W6010908 hasConceptScore W6010908C134018914 @default.
• W6010908 hasConceptScore W6010908C163235415 @default.
• W6010908 hasConceptScore W6010908C170493617 @default.
• W6010908 hasConceptScore W6010908C180526460 @default.
• W6010908 hasConceptScore W6010908C185592680 @default.
• W6010908 hasConceptScore W6010908C24998067 @default.
• W6010908 hasConceptScore W6010908C2777427919 @default.

• next