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- W60360422 abstract "Experiments were designed to investigate the role of nitric oxide (NO) in the expression of stem cell specific marker Oct-4 and the differentiation of bone marrow stem cells into endothelial cells in vitro. The expression of Oct-4 in the stem cells with or without NOS inhibitor L-NAME (100 uM and 1000 uM) was measured with RT-PCR. The Oct-4 level in the cells without L-NAME was 24.2„b2.0% (n=5) of ES cells. It was not changed in the cells treated with L-NAME (25.6„b3.6%, n=5; p>0.05). The proliferation rate was also not affected by L-NAME after 48 hours incubation. To evaluate the effect of NO on the differentiation in vitro, the stem cells were induced to differentiate into endothelial cells. The course of cell differentiation into endothelial cells was monitored with the endothelial cell marker vWF. The cells started to express vWF at day 7 and reached maximum level at day 14 after the initiation of differentiation. Neither the magnitude nor the time course of vWF expression was changed in the cells treated with L-NAME. These data indicate that the biological behavior of the mouse bone marrow stem cells is independent of NOS inhibition in vitro." @default.
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- W60360422 date "2006-03-01" @default.
- W60360422 modified "2023-10-18" @default.
- W60360422 title "Inhibition of Nitric Oxide Synthase Does Not Change Oct‐4 Expression or Differentiation of Bone Marrow Stem Cells into Endothelial Cells in vitro" @default.
- W60360422 doi "https://doi.org/10.1096/fasebj.20.5.a1126-c" @default.
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