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- W60439265 abstract "This chapter focuses on size distribution and β2-microglobulin content of human natural killer (NK) cells analyzed by fluorescence-activated cell sorting (FACS). In a study described in the chapter, normal peripheral blood mononuclear cells were isolated by Isopaque–Ficoll method and stained with a FITC-conjugated rabbit anti-β2-M antiserum. The cells were subsequently processed on a FACS II machine. In the chapter, the dot plot screen of the FACS II from a typical experiment is shown, each cell being represented by a dot giving its size (or forward light scatter, x-axis) and the fluorescence intensity (y-axis). Both lymphocytes and monocytes—as verified by Giemsa-stained smears—can be delineated and lymphocytes can be further subdivided into four lymphocyte subsets according to their β2-M content and size. The FACS was set up to sort the 20% least and the 20% most fluorescing cells, and it was found that cells with low β2-M content, irrespective of size, always exhibited lower NK than the high β2-M containing cells and that IFN-inducible NK cells moreover were found in the β2-M rich cell fractions, indicating that the association between β2-M and NK might be stronger than that between NK and cell size." @default.
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- W60439265 date "1982-01-01" @default.
- W60439265 modified "2023-10-05" @default.
- W60439265 title "SIZE DISTRIBUTION AND β2-MICROGLOBULIN CONTENT OF HUMAN NATURAL KILLER CELLS AS ANALYZED BY FLUORESCENCE ACTIVATED CELL SORTING (FACS)" @default.
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- W60439265 doi "https://doi.org/10.1016/b978-0-12-341360-4.50021-4" @default.
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