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- W604601844 abstract "Excised runner apices from strawberry which were floated on 0.5 ml of freezing solution including 3% sucrose and 10 to 16% DMSO in a spitz tube were immersed in liquid nitrogen (cooling rate: 420°C/min) after prefreezing to - 40°C and were then rewarmed rapidly. Only about 15% of these treated apices initiated shoot development in the light on MS cultural medium. To increase the survival rate of the apices frozen to the tempera ture of liquid nitrogen, excised runner apices on a coverglass were rapidly frozen from room temperature in liquid nitrogen (cooling rate: 10 5 °C/min) after prefreezing to - 20 to - 30°C in the presence of 16% DMSO and were then rewarmed very rapidly by direct immersion in MS cultural solution at 40°C. About 60 to 80% of the runner apices frozen to -196°C developed normal shoots in the light on the MS cultural medium." @default.
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- W604601844 date "1979-03-30" @default.
- W604601844 modified "2023-09-26" @default.
- W604601844 title "Development of a Whole Plant from Excised Strawberry Runner Apex Frozen to -196℃" @default.
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