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- W60488789 abstract "Xenopus oocytes were injected with poly(A{sup +}) RNA prepared from rat kidney. After a four-day incubation, {sup 86}Rb uptake was measured in the presence of 500 {mu}M ouabain, and 1 mM Ba{sup ++}. Na{sup +} (83 mM) stimulated {sup 86}Rb uptake by over 3-fold compared to the choline control. Bumetanide (10 {mu}M) completely inhibited the Na{sup +} stimulation. Rat kidney poly(A{sup +})RNA was size-fractionated either by acid urea agarose gel electrophoresis or by sucrose density gradient ultracentrifugation. The fractions were injected into oocytes. Bumetanide-inhibitable {sup 86}Rb uptake was induced in the oocytes by only one of the fractions in either case - a high molecular weight fraction {ge}7 KB. Conclusion: Xenopus oocytes will express messenger RNA encoding the rat renal Na{sup +}, K{sup +}, Cl{sup {minus}}-cotransporter. This mRNA has been further purified by size-based methods and was detected in a single high molecular weight fraction." @default.
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- W60488789 date "1990-02-26" @default.
- W60488789 modified "2023-09-22" @default.
- W60488789 title "Expression of a Na sup + , K sup + , Cl sup minus -cotransporter in xenopus oocytes" @default.
- W60488789 hasPublicationYear "1990" @default.
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