Matches in SemOpenAlex for { <https://semopenalex.org/work/W60508758> ?p ?o ?g. }
Showing items 1 to 60 of
60
with 100 items per page.
- W60508758 abstract "Cytochrome c peroxidase (CcP) is a yeast mitochondrial enzyme that catalyzes the reduction of hydrogen peroxide to water by ferrocytochrome c. The mechanism involves complex formation between cytochrome c and the enzyme. The driving force for complex formation between cytochrome c and CcP is largely electrostatic in nature. Both 1:1 and 2:1 cytochrome c/CcP complexes have been observed. In order to map the interaction between cytochrome c and CcP in solution, eleven different charge-reversal mutants have been constructed by converting either aspartate or glutamate residues on the surface of CcP to lysine residues. All of the aspartate and glutamate residues are in the vicinity of the cytochrome c binding site identified by x-ray crystallography (Pelletier and Kraut (1992) Science 258, –1755). The effect of these charge-reversal mutations on the steady-state kinetic properties of CcP catalysis has been investigated. Four mutations cause large increases in the Michaelis constant for the cytochrome c interaction with CcP: D34K, D37K, E118K, and E290K. This research is supported in part by the NIH through grant R15 GM59740." @default.
- W60508758 created "2016-06-24" @default.
- W60508758 creator A5006293009 @default.
- W60508758 creator A5019356414 @default.
- W60508758 creator A5087172093 @default.
- W60508758 date "2007-04-01" @default.
- W60508758 modified "2023-09-25" @default.
- W60508758 title "Effect of Charge‐Reversal Mutations on the Catalytic Activity of Cytochrome c Peroxidase" @default.
- W60508758 doi "https://doi.org/10.1096/fasebj.21.5.a272-d" @default.
- W60508758 hasPublicationYear "2007" @default.
- W60508758 type Work @default.
- W60508758 sameAs 60508758 @default.
- W60508758 citedByCount "0" @default.
- W60508758 crossrefType "journal-article" @default.
- W60508758 hasAuthorship W60508758A5006293009 @default.
- W60508758 hasAuthorship W60508758A5019356414 @default.
- W60508758 hasAuthorship W60508758A5087172093 @default.
- W60508758 hasBestOaLocation W605087581 @default.
- W60508758 hasConcept C133166286 @default.
- W60508758 hasConcept C162008176 @default.
- W60508758 hasConcept C181199279 @default.
- W60508758 hasConcept C185592680 @default.
- W60508758 hasConcept C2776016237 @default.
- W60508758 hasConcept C2780768313 @default.
- W60508758 hasConcept C28859421 @default.
- W60508758 hasConcept C29311851 @default.
- W60508758 hasConcept C515207424 @default.
- W60508758 hasConcept C55493867 @default.
- W60508758 hasConcept C71240020 @default.
- W60508758 hasConceptScore W60508758C133166286 @default.
- W60508758 hasConceptScore W60508758C162008176 @default.
- W60508758 hasConceptScore W60508758C181199279 @default.
- W60508758 hasConceptScore W60508758C185592680 @default.
- W60508758 hasConceptScore W60508758C2776016237 @default.
- W60508758 hasConceptScore W60508758C2780768313 @default.
- W60508758 hasConceptScore W60508758C28859421 @default.
- W60508758 hasConceptScore W60508758C29311851 @default.
- W60508758 hasConceptScore W60508758C515207424 @default.
- W60508758 hasConceptScore W60508758C55493867 @default.
- W60508758 hasConceptScore W60508758C71240020 @default.
- W60508758 hasFunder F4320332161 @default.
- W60508758 hasIssue "5" @default.
- W60508758 hasLocation W605087581 @default.
- W60508758 hasOpenAccess W60508758 @default.
- W60508758 hasPrimaryLocation W605087581 @default.
- W60508758 hasRelatedWork W1979324186 @default.
- W60508758 hasRelatedWork W1993515669 @default.
- W60508758 hasRelatedWork W2012727516 @default.
- W60508758 hasRelatedWork W2013230341 @default.
- W60508758 hasRelatedWork W2017369432 @default.
- W60508758 hasRelatedWork W2030251511 @default.
- W60508758 hasRelatedWork W2050845517 @default.
- W60508758 hasRelatedWork W2082631749 @default.
- W60508758 hasRelatedWork W2947062577 @default.
- W60508758 hasRelatedWork W94736568 @default.
- W60508758 hasVolume "21" @default.
- W60508758 isParatext "false" @default.
- W60508758 isRetracted "false" @default.
- W60508758 magId "60508758" @default.
- W60508758 workType "article" @default.