Matches in SemOpenAlex for { <https://semopenalex.org/work/W605698293> ?p ?o ?g. }
Showing items 1 to 63 of
63
with 100 items per page.
- W605698293 abstract "FAN1/MTMR15 (Fanconi anemia-associated nuclease 1 / Myotubularin-related protein 15) is a protein originally identified from a set of size-fractionated human brain cDNA libraries coding for large proteins in vitro (Nagase et al., 1999). FAN1 is widely conserved across eukaryotes, with the notable exception of S. cerevisiae (Smogorzewska et al., 2010; MacKay et al., 2010; Kratz et al., 2010; Liu et al., 2010; Shereda et al., 2010). Recent work has shown that FAN1 is a novel component of the Fanconi Anemia repair DNA pathway in higher eukaryotes (Smogorzewska et al., 2010; MacKay et al., 2010; Kratz et al., 2010; Yoshikiyo et al., 2010; Liu et al., 2010; Shereda et al., 2010).My work presents a biochemical and genetic characterisation of the FAN1 Schizosaccharomyces pombe ortholog Fan1. I show that, in contrast with the situation in higher eukaryotes, Fan1 in S. pombe does not strongly interact with components of the mismatch repair pathway. The disruption of fan1 causes a mild sensitivity to interstrand cross-linking agents, dramatically augmented by the concomitant deletion of the nuclease Pso2, suggesting a role for Fan1 in the resolution of DNA interstrand cross-links. Further genetic interactions are explored by use of an automated high-throughput screen, where a non-epistatic relationship is found with Pli1, a component of the SUMOylation pathway. Finally, I show that three conserved residues in the VRR_nuc nuclease domain are required for Fan1 activity in DNA repair. Taken together, the data presented points at a role for S. pombe Fan1 in the resolution of adducts created by DNA interstrand cross-linking agents." @default.
- W605698293 created "2016-06-24" @default.
- W605698293 creator A5043678085 @default.
- W605698293 date "2011-11-07" @default.
- W605698293 modified "2023-09-26" @default.
- W605698293 title "Novel insights into the DNA interstrand cross-link repair in Schizosaccharomyces pombe: characterisation of Fan1 through standard and high-throughput genetic analysis" @default.
- W605698293 hasPublicationYear "2011" @default.
- W605698293 type Work @default.
- W605698293 sameAs 605698293 @default.
- W605698293 citedByCount "0" @default.
- W605698293 crossrefType "dissertation" @default.
- W605698293 hasAuthorship W605698293A5043678085 @default.
- W605698293 hasConcept C104317684 @default.
- W605698293 hasConcept C134935766 @default.
- W605698293 hasConcept C143425029 @default.
- W605698293 hasConcept C2776653068 @default.
- W605698293 hasConcept C2777271071 @default.
- W605698293 hasConcept C2777576037 @default.
- W605698293 hasConcept C2778124228 @default.
- W605698293 hasConcept C2779429346 @default.
- W605698293 hasConcept C54355233 @default.
- W605698293 hasConcept C552990157 @default.
- W605698293 hasConcept C86803240 @default.
- W605698293 hasConcept C95444343 @default.
- W605698293 hasConceptScore W605698293C104317684 @default.
- W605698293 hasConceptScore W605698293C134935766 @default.
- W605698293 hasConceptScore W605698293C143425029 @default.
- W605698293 hasConceptScore W605698293C2776653068 @default.
- W605698293 hasConceptScore W605698293C2777271071 @default.
- W605698293 hasConceptScore W605698293C2777576037 @default.
- W605698293 hasConceptScore W605698293C2778124228 @default.
- W605698293 hasConceptScore W605698293C2779429346 @default.
- W605698293 hasConceptScore W605698293C54355233 @default.
- W605698293 hasConceptScore W605698293C552990157 @default.
- W605698293 hasConceptScore W605698293C86803240 @default.
- W605698293 hasConceptScore W605698293C95444343 @default.
- W605698293 hasLocation W6056982931 @default.
- W605698293 hasOpenAccess W605698293 @default.
- W605698293 hasPrimaryLocation W6056982931 @default.
- W605698293 hasRelatedWork W1943069336 @default.
- W605698293 hasRelatedWork W1968128183 @default.
- W605698293 hasRelatedWork W1988436532 @default.
- W605698293 hasRelatedWork W2034353458 @default.
- W605698293 hasRelatedWork W2035405497 @default.
- W605698293 hasRelatedWork W2059753431 @default.
- W605698293 hasRelatedWork W2088555353 @default.
- W605698293 hasRelatedWork W2096975251 @default.
- W605698293 hasRelatedWork W2098383858 @default.
- W605698293 hasRelatedWork W2103590738 @default.
- W605698293 hasRelatedWork W2105650420 @default.
- W605698293 hasRelatedWork W2129215634 @default.
- W605698293 hasRelatedWork W2134844472 @default.
- W605698293 hasRelatedWork W2137562090 @default.
- W605698293 hasRelatedWork W2149156322 @default.
- W605698293 hasRelatedWork W2167954691 @default.
- W605698293 hasRelatedWork W2221958189 @default.
- W605698293 hasRelatedWork W2292864272 @default.
- W605698293 hasRelatedWork W287874540 @default.
- W605698293 hasRelatedWork W94095845 @default.
- W605698293 isParatext "false" @default.
- W605698293 isRetracted "false" @default.
- W605698293 magId "605698293" @default.
- W605698293 workType "dissertation" @default.