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- W607647515 abstract "Amphotericin B is a polyene macrolide antibiotic known to induce cation-selective pore formation in biological cell membranes. Application of amphotericin B damages different type of cells and causes cell death. We investigated the mechanism of amphotericin B induced cell death and found that Cl− ion permeability was essential for the effect of amphotericin B. We applied amphotericin B to Xenopus oocytes and calculated cell volume using imaging system. We found that 10mg/ml of amphotericin B made oocytes increase cell volume above 35% and then oocytes ruptured within three hours. Replacement of extra-cellular Cl− by gluconate− completely blocked amphotericin B induced volume increase and cell death in oocytes. Replacement of extra-cellular Na+ by K+ had no effect. We found that two lysosomal proton pump blocker (0.1mM N-ethylmaleimide (NEM), 0.5mM chloroquine) or chloride channel blocker (0.5mM DIDS) inhibited amphotericin B induced cell volume increase and membrane rupture. Two electrode voltage-clamp study of Xenopus oocytes was also performed. Amphotericin B induced cation selective conductance increase and both NEM and chloroquine had no effect on amphotericin B induced conductance increase. These evidences suggest that the influx of Cl− ions through endogenous Cl− channels, caused by amphotericin B induced cationic permeability increase, may induce lysosomal acidification and then induced damage of oocyte cell membrane." @default.
- W607647515 created "2016-06-24" @default.
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- W607647515 date "2006-03-01" @default.
- W607647515 modified "2023-10-18" @default.
- W607647515 title "Volume increase and membrane rupture induced by amphotericin B in Xenopus oocytes" @default.
- W607647515 doi "https://doi.org/10.1096/fasebj.20.5.a1463-c" @default.
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