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- W608619096 abstract "The present paper reports on the purification and characterization of an extracellular keratinase (KERQ7) newly purified from Bacillus tequilensis Q7. Pure protein was obtained after ammonium sulfate fractionation (30-60%), followed by Mono S Sepharose cation-exchange chromatography. MALDI-TOF/MS analysis revealed that the purified enzyme was a monomer with a molecular mass of 28,355.07-Da. The sequence of the 21 N-terminal residues of KERQ7 showed high homology with those of Bacillus keratinases. Optimal activity was achieved at pH 7 and 30°C. KERQ7 was completely inhibited by PMSF and DFP, which suggests that it belongs to the serine keratinase family. KERQ7 displayed higher levels of hydrolysis and catalytic efficiency than Basozym(®) CS 10, Koropon(®) SC 5K, and Pyrase(®) 250 MP. The kerQ7 gene encoding KERQ7 was cloned, sequenced, and expressed in Escherichia coli BL21(DE3)pLysS. The biochemical properties of the extracellular purified recombinant enzyme (rKERQ7) were similar to those of native KERQ7. The deduced amino acid sequence showed strong homology with other Bacillus keratinases. The highest sequence identity value (97%) was obtained with KERUS from Brevibacillus brevis US575, with only 7 aa of difference. These properties make KERQ7 a potential promising and eco-friendly enzymatically enhanced process for animal hide bating in the leather processing industry." @default.
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- W608619096 date "2015-08-01" @default.
- W608619096 modified "2023-09-24" @default.
- W608619096 title "A novel keratinase from Bacillus tequilensis strain Q7 with promising potential for the leather bating process" @default.
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- W608619096 doi "https://doi.org/10.1016/j.ijbiomac.2015.05.038" @default.
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