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- W60872095 abstract "Induction of Heme Oxygenase-1 (HO-1) in the renal medulla increases CO and bilirubin production and decreases Angiotensin II (Ang II)-mediated superoxide production. The goal of this study was to determine the relative importance of increases in CO and bilirubin to the antioxidant effects of HO-1 induction in cultured thick ascending loop of Henle (TALH) cells. The effects of CO and bilirubin were separated by using small interfering RNAs (siRNAs) targeted to biliverdin reductase (BVR) which is the cellular enzyme responsible for the conversion of biliverdin to bilirubin. Treatment of cultured TALH with BVR siRNA (100 nM) resulted in a 70–90% decrease in the level of BVR protein and decreased cellular bilirubin levels from 46 ± 5 to 23 ± 4 nM (n=4). Next, we determined the effects of inhibition of BVR on Ang II-mediated superoxide production in cultured TALH cells. Superoxide production to Ang II (10−9 M) was determined using dihydroethidium (DHE) staining followed by confocal microscopy and expressed as percent of control staining. Treatment of TALH cells with BVR siRNA (50, 100 nM) resulted in a significant increase in Ang II-mediated superoxide production and averaged 109 ± 4, 145 ± 12, 164 ± 7%, respectively, in non-targeting vs. 50 vs.100 nM BVR siRNA (n=4). These results demonstrate that decreased levels of cellular bilirubin increase Ang II-mediated superoxide production in TALH cells. These studies were supported by a grant from the American Heart Association-Southeast Affiliate." @default.
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- W60872095 date "2008-03-01" @default.
- W60872095 modified "2023-09-26" @default.
- W60872095 title "Inhibition of biliverdin reductase increases Angiotensin II mediated superoxide production in thick ascending loop of Henle cells" @default.
- W60872095 doi "https://doi.org/10.1096/fasebj.22.1_supplement.1159.4" @default.
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