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- W609319265 abstract "Photorhabdus temperata is a Gram negative entomopathogenie bacterium of the Enterobactericae. P. Temperata lives in symbiosis in the intestine of nematodes of the family, Heterorhabditae, which, in turn, are capable of penetrating into the interior of insect larvae. Within the insect, nematodes release the bacteria which rapidly multiply, killing the larva then hydrolysing the internai structure, providing ideal conditions for nematode reproduction and development. Finally, nematodes and bacteria re-enter a symbiotic interaction and they leave 1the cadaver in search of a new hosto P. Temperata exhibits two distinct phenotypic phase variants in the laboratory, termed phase 1 and II. ln an attempt to elucidate the factors and mechanisms implicated in phase variation we initially performed RAP PCR (RNA fingerprint by Arbitrarily Primed PCR) in order to identify genes specifically expressed in the two phase variant forms of P. Temperata in laboratory LB medium. Several genes were identified during this study, including two clones with homology to a major subunit and one similar to a minor subunit of mannose resistant fimbriae encoded by MR/P genes of Proteus mirabilis. Fimbriae typically play an important Tale in virulence and thus we continued with a more detailed study of these structures in P. Temperata. Concomitant with the above investigations, we also studied a zinc metalloprotease, : PrtA, secreted to the culture medium by P. Tempe rata. PrtA is a member of the RTX (Repeats. 1 in Toxin) family. Finally, a study was undertaken using the technique of genomic DNA subtractive C hybridisation between two species of Photorhabdus, P. Temperata K122 and the closely related P. Luminescens W14. Despite having a similar pathology in insect infections, they ~ exhibit many different phenotypic traits, leading to the hypothesis that, although they may ~ employ many similar virulence factors, some may be species specific." @default.
- W609319265 created "2016-06-24" @default.
- W609319265 creator A5026593210 @default.
- W609319265 date "2003-01-01" @default.
- W609319265 modified "2023-09-23" @default.
- W609319265 title "Identification et analyse de facteurs de virulence d'une bactérie entomopathogène, Photorhabdus temperata" @default.
- W609319265 hasPublicationYear "2003" @default.
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