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- W610021544 abstract "[Objective] The aim was to study the structural characteristics of xyloglucan endotransglycosylase/ hydrolase(XTH) gene in ethylene-insensitive feverfew.[Method] The total RNA was extracted from Chrysanthemum × morifolium petal using Trizol reagent,and the cDNA fragment of XTH gene was cloned by RT-PCR and T/A cloning,and then analyzed by agarose gel electrophresis and sequencing.[Result] The cloned cDNA sequence was 911 bp.It was predicted to encode a polypeptide of 293 amino acids and had seven active sites of XTH family,and then named as CmXTH(gene accession number HM752243).In addition,the BLAST analysis showed that the deduced amino acid sequence of CmXTH showed high homology with other 19 chosen plant XTHs.Among these,CmXTH had closer genetic relationship with Gerbera hybrid cultivar XET,Solanum lycopersicum XTH7,whereas had relatively distant relationship with Populus euparatica XET,Fragaria ananassa XET1,Actinidia deliciosa XET,etc.[Conclusion] The cloned fragment was certainly cDNA sequence of XTH gene,which was associated with the petal growth and senescence in Chrysanthemum × morifolium.The study lays foundation for further studying the structure and biological functions of XTH genes as well as the growth of Chrysanthemum×morifolium petals." @default.
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- W610021544 date "2012-01-01" @default.
- W610021544 modified "2023-09-23" @default.
- W610021544 title "Cloning and analysis of the cDNA sequence of XTH gene from Chrysanthemum morifolium petal." @default.
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