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- W611873677 abstract "提取Trichinella nativa（T.nativa）肌幼虫的总RNA,用RT-PCR方法扩增出了编码T.nativa 49 Ku ES蛋白的结构基因.基因克隆后测序,序列测定结果表明：目的基因TNPG长度为951 bp,核苷酸序列同已发表的Trichinella spiralis（T.spiralis）相应的序列P49同源性为97.68%,所推导的氨基酸序列同源性为95.24%.将目的基因TNPG插入到原核表达载体pET-30a的BamH I酶切位点处,并转化到感受态表达菌中进行诱导表达.结果显示TNPG在原核表达菌BL-21中获得了高效表达,表达产物为40.8 Ku的融合蛋白,表达量达到菌体总蛋白的22.8%.通过Western blot分析,表达产物可以被小鼠T.nativa和T.spiralis阳性血清以及它们的中国地理株的小鼠血清特异性识别." @default.
- W611873677 created "2016-06-24" @default.
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- W611873677 date "2007-01-01" @default.
- W611873677 modified "2023-09-23" @default.
- W611873677 title "本地毛形线虫49 Ku ES 蛋白结构基因的分子克隆及原核表达" @default.
- W611873677 hasPublicationYear "2007" @default.
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