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- W61281966 abstract "To explore the expression of cadherin isoforms in cultured human gastric carcinoma cells and its regulation.The expressions of cell adhesion molecules (including E-cadherin, N-cadherin, alpha-catenin, beta-catenin) and cadherin transcription factors including snail, slug and twist were determined by reverse transcriptase-polymerase chain reaction(RT-PCR), immunoblotting and immunofluorescence in SV40-immortalized human gastric cell line Ges-1 and human gastric cancer cell lines MGC-803, BGC-823 and SGC-7901.All cell lines expressed N-cadherin, but not E-cadherin. N-cadherin immunofluorescence was detected at cell membranous adherents junctions where co-localization with immunofluorescent staining of inner surface adhesion proteins alpha- and beta-catenins was observed. The transformed Ges-1 and gastric cancer cell lines all expressed transcription factors (snail, slug and twist) which inhibited the expression of E-cadherin and triggered epithelial-mesenchymal transformation.Cadherin isoforms can change from E-cadherin to N-cadherin in transformed human gastric cancer cells, which is associated with intracellular events of stomach carcinogenesis and high expression of corresponding transcription factors." @default.
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- W61281966 date "2006-01-01" @default.
- W61281966 modified "2023-09-27" @default.
- W61281966 title "Conversion of cadherin isoforms in cultured human gastric carcinoma cells" @default.
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- W61281966 doi "https://doi.org/10.3748/wjg.v12.i6.966" @default.
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