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• W61293054 abstract "The colour difference between yellow- and red-fleshed Carica papaya L. is the accumulation of an additional carotenoid, lycopene, in red-fleshed fruit. In this thesis I have used a combination of gene discovery, gene expression and metabolite profiling strategies in search of candidate genes that are responsible for papaya colour and aroma traits. A particular focus on papaya fruit colour associated genes resulted in the identification of a candidate gene whose expression patterns correlated with the desirable red flesh colour. Gene discovery involved the generation of 1052 expressed sequence tags from fruit cDNA libraries of the yellow-fleshed 1B and red-fleshed Tainung hybrid varieties. Sequence searches against annotated plant databases identified numerous softeningcolour- and aroma-associated genes. Genes associated with papaya carotenogenesis were also identified using a method involving transformation of carotenoidaccumulating Escherichia coli strains with papaya cDNA libraries. Oligonucleotide microarray analysis was used to measure expression profiles of 1021 papaya genes, including newly discovered fruit-expressed sequence tags and papaya mRNA sequences from the public NCBI non-redundant nucleotide sequence database. Microarray analysis identified global fruit mesocarp gene expression differences during immature green, mature green and ¼ skin colour break fruit developmental stages in the yellow-fleshed 1B. In a colour pathway focused experiment, expression of selected papaya genes encoding enzymatic steps of the isoprenoid biosynthesis pathway were compared across fruit development stages in the colour-contrasted Tainung and 1B varieties. Metabolic profiling of carotenoids across the same fruit development stages in Tainung and 1B varieties was performed. Examination of both the gene expression and metabolite profiles helped to identify a candidate gene likely responsible for the biosynthesis bottleneck causing lycopene accumulation uniquely in red-fleshed papaya. A chromoplast-specific lycopene β-cyclase appears to be responsible for the colour variation. The gene, lcy-β2, was discovered using a degenerate primer approach in conjunction with rolling circle rapid amplification of cDNA ends (RCA-RACE) and found to exhibit β-cyclase activity. Comparison of the gene sequence of Tainung and 1B identified one single nucleotide polymorphism (SNP) in the coding region and it showed perfect segregation between the yellow and red varieties. Development of a SNP marker for papaya flesh colour is discussed." @default.
• W61293054 created "2016-06-24" @default.
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• W61293054 date "2008-02-01" @default.
• W61293054 modified "2023-09-27" @default.
• W61293054 title "Molecular Genetics of Carotenoid Production in Papaya Fruit" @default.
• W61293054 hasPublicationYear "2008" @default.
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