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- W61491315 abstract "[Objective] To identify the characteristics of nrDNA-ITS from the leaf of famous-region drug Codonopsis tangshen Oliv.It will provide molecular evidence to distinguish the Codonopsis tangshen Oliv.[Method]Direct PCR sequencing was used to detect the homology of nrDNA-ITS seqμence of Codonopsis tangshen Oliv,and analyze its sequence composition. [Result] The ratio of OD260/OD280 of genome DNA absorption value in samples extracted by improved CTAB was 1.8-2.0,DNA strips were clear and tidy,no obvious tail phenomenon,indicating that purity and quality of DNA samples was better,met the test requirements. NrDNA-ITS sequences universal primers were used to PCR amplification reactions,the expected double-stranded product of about 800 bp were obtained. After sequencing,it revealed that the Nucleotide sequence length of Codonopsis tangshen Oliv. nrDNA-ITS1 sequence was 260 bp,its G + C contents was 52%,the nucleotide sequence length of nrDNA-ITS2 sequence was 239 bp,its G+C contents was 60%; Blast comparative analysis in GenBank confirmed that it belonged to Codonopsis plants of nrDNA-ITS sequences,and had been submitted to GenBank,registration No. was GQ906566. [Conclusion] DNA sequencing can provide an accurate and reliable mean for the identification of the origin and genuineness of C. tangshen Oliv,which also can provide the basic information for molecular identification of C. tangshen Oliv." @default.
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- W61491315 date "2010-01-01" @default.
- W61491315 modified "2023-09-23" @default.
- W61491315 title "Analysis of nrDNA-ITS gene sequence of famous-region drug Codonopsis tangshen Oliv." @default.
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