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- W616185893 abstract "A metagenomic library of uncultured bacteria from bovine rumen fluid content was constructed,which contained 12 000 clones and the capacity of inserted DNA,of which was 4.2×10~8 bp.Nine clones expressing β-glucosidase activity were isolated and a clone designated pGXN1009 was subcloned and a putative β-glucosidase gene was localised to a 4.3 kb fragment.Sequencing of this 4.3 kb DNA fragment revealed that there is a β-glucosidase gene umbgl3A with an ORF of 1956 bp,encoding product shared 63% identity and 79%similarity with a beta-glucosidase(GenBank NO:AAX16378.1) of uncultured murine large bowel bacterium BAC 31B,and shared 50% identity and 67%similarity with a beta-glucosidase-related glycosidases (GenBank NO:ZP-00308419.1) of Cytophaga hutchinsonii.Umbgl3A possessed a domain of glycosyl hydrolase family 3 and a domain of glycosyl hydrolase family 3C.Phylogentic analysis of Umbgl3A indicated umbgl3A may come from a species of Cytophaga." @default.
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- W616185893 date "2005-01-01" @default.
- W616185893 modified "2023-09-25" @default.
- W616185893 title "Cloning and identification a β-glucosidase gene umbgl3A of uncultured bacteria from bovine rumen." @default.
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