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• W617159298 abstract "We report a highly sensitive method for the electrochemical detection of genomic DNA, based on the employment of two sub-micron oligonucleotide labels - one for magnetic collection and the other for voltammetric detection - and their incorporation onto a stem loop DNA probe. The magnetic label consists of a latex particle of mean diameter 441±6 nm, bearing magnetic Fe3O4 particles and approx. 3.5×10(5) anti-DIG antibodies. The voltammetric label is a hollow polyelectrolyte shell containing approx. 1.0×10(11) Au atoms in the form of well dispersed Au nanoparticles. A DIG tag on one arm of the stem loop enables binding to the magnetic label, while a thiol tag on the other arm enables attachment to the Au nanoparticles. Due to steric hindrance from the two relatively large labels, attachment of both moieties is dependant on target-probe hybridisation straightening the loop. Once attached, sensitive DNA measurement is facilitated by magnetic collection of the DNA into a small volume and by the high quantity of Au atoms available for detection. Using differential pulse anodic stripping voltammetry we calibrated a 30 mer sequence common to 71 strains of Escherichia coli across the concentration range from 0.1 aM to 100 pM with a LOD of 1.8 aM. Three strains of E. coli, BL 21, ATCC8739, O157:H7, when spiked into UHT milk and fermented palm juice, could be detected with LODs of approx. 2-4 CFU mL(-1) in an assay time of approx. 140 min." @default.
• W617159298 created "2016-06-24" @default.
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• W617159298 date "2015-11-01" @default.
• W617159298 modified "2023-10-14" @default.
• W617159298 title "Highly sensitive electrochemical detection of genomic DNA based on stem loop probes structured for magnetic collection and measurement via metalised hollow polyelectrolyte shells" @default.
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• W617159298 doi "https://doi.org/10.1016/j.bios.2015.05.068" @default.
• W617159298 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26067330" @default.
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