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- W6198744 abstract "The Structure of the MAP2K MEK6 Reveals an Autoinhibitory Dimer Xiaoshan Min,1,4 Radha Akella,1 Haixia He,1 John M. Humphreys,1 Susan E. Tsutakawa,2 Seung- Jae Lee,1 John A. Tainer,2 Melanie H. Cobb,3 and Elizabeth J. Goldsmith1,* 1Department of Biochemistry, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75390-8816, USA 2Lawrence Berkeley National Lab, 1 Cyclotron Road, Berkeley, CA 94720, USA 3Department of Pharmacology, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75390-9041, USA 4Present address: Amgen, One Amgen Center Drive, Thousand Oaks, CA 91320-1799, USA *Correspondence: elizabeth.goldsmith@utsouthwestern.edu SUMMARY MAP2Ks are dual-specificity protein kinases functioning at the center of three-tiered MAP kinase modules. The structure of the kinase domain of the MAP2K MEK6 with phosphorylation site mimetic aspartic acid mutations (MEK6/DN/DD) has been solved at 2.3 A° resolution. The structure reveals an autoinhibited elongated ellipsoidal dimer. The enzyme adopts an inactive conformation, based upon structural queues, despite the phosphomimetic mutations. Gel filtration and small- angle X-ray scattering analysis confirm that the crystallographically observed ellipsoidal dimer is a feature of MEK6/DN/ DD and full-length unphosphorylated wild-type MEK6 in solution. The interface includes the phosphate binding ribbon of each subunit, part of the activation loop, and a rare ‘‘arginine stack’’ between symmetry-related arginine residues in the N-terminal lobe. The autoinhibited structure likely confers specificity on active MAP2Ks. The dimer may also serve the function in unphosphorylated MEK6 of preventing activation loop phosphorylation by inappropriate kinases." @default.
- W6198744 created "2016-06-24" @default.
- W6198744 creator A5021430416 @default.
- W6198744 date "2010-02-09" @default.
- W6198744 modified "2023-09-28" @default.
- W6198744 title "The Structure of the MAP2K MEK6 Reveals an Autoinhibitory Dimer" @default.
- W6198744 hasPublicationYear "2010" @default.
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