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- W620062489 abstract "In this study, we developed a CdSe/ZnS quantum dot (QD)-based immunoassay for use in determining the presence of progesterone (P4) in human serum. Hydrophilic QDs were conjugated to anti-progesterone antibody (P4Ab) via ethyl-3-(dimethylaminopropyl)carbodiimide (EDC) and N-hydroxysuccinimide (NHS) as coupling reagents. After purification, the P4Ab-QD conjugates were immobilized onto the wells of a 96-well microtiter plate, and a direct-binding immunoassay based on the binding of P4 to immobilized P4Ab-QD conjugates had a detection limit of 0.21 ng/ml and a sensitivity of 1.37 ng/ml, with a linear range of 0.385 to 4.55 ng/ml. The proposed immunoassay was successfully used to determine the P4 concentration in real human serum, and the results showed a good correlation with the accredited radioimmunoassay (RIA)." @default.
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- W620062489 date "2015-08-01" @default.
- W620062489 modified "2023-10-18" @default.
- W620062489 title "Simple and sensitive progesterone detection in human serum using a CdSe/ZnS quantum dot-based direct binding assay" @default.
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- W620062489 doi "https://doi.org/10.1016/j.ab.2015.05.001" @default.
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