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- W620065151 abstract "To compare biofilm formation in trimethoprim/sulfamethoxazole (SXT)-susceptible Escherichia coli (E. coli) (SSEC) and SXT-resistant E. coli (SREC) isolated from patients with urinary tract infections, and study the motile ability and physical characteristics of the isolates. A total of 74 E. coli isolates were tested for antimicrobial susceptibility with the disc diffusion assay. Based on the SXT-susceptibility test, the E. coli isolates were divided into SSEC (N = 30) and SREC (N = 44) groups. All E. coli isolates were examined for motile ability by using a motility test medium, and for checking biofilm formation a scanning electron microscope was used. Bacterial colony size was measured with a vernier caliper and bacterial cell length was measured under a light microscope. The bacterial growth rate was studied by plotting the cell growth (absorbance) versus the incubation time. The frequencies of non-motility and biofilm formation in the SREC group were significantly higher than that in the SSEC group (P < 0.01). The SREC bacterial cell length was shorter than that in the SSEC group [(1.35 ± 0.05) vs. (1.53 ± 0.05) μm, P < 0.05)], whereas the bacterial colony size and mid-log phase of the growth curve were not significantly different. The present study indicated that biofilm formation and phenotypic change of uropathogenic E. coli can be attributed to the mechanism of E. coli SXT resistance." @default.
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- W620065151 date "2015-06-01" @default.
- W620065151 modified "2023-10-17" @default.
- W620065151 title "Biofilm formation in trimethoprim/sulfamethoxazole-susceptible and trimethoprim/sulfamethoxazole-resistant uropathogenic Escherichia coli" @default.
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- W620065151 doi "https://doi.org/10.1016/j.apjtb.2015.03.006" @default.
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