Matches in SemOpenAlex for { <https://semopenalex.org/work/W620671009> ?p ?o ?g. }
- W620671009 abstract "Amino-acid radical enzymes have been studied extensively for 30 years but the experimental barriers to determine the thermodynamic properties of their key radical cofactors are so challenging that only a handful of reports exist in the literature. This is a major drawback when trying to understand the long-range radical transfer and/or catalytic mechanisms of this important family of enzymes. Here this issue is addressed by developing a library of well-structured model proteins specifically designed to study tyrosine and tryptophan radicals. The library is based on a 67-residue three-helix bundle (α3W) and a 117-residue four-helix bundle (α4W). α3W and α4W are single-chain and uniquely structured proteins. They are redox inert except for a single radical site (position 32 in α3W and 106 in α4W). Papers I and II describe the design process and the protein characteristics of α4W as well as a voltammetry study of its unique tryptophan. Paper III and V describe two projects based on α3C, which is a Trp-32 to Cys-32 variant of α3W. In Paper III we use α3C to investigate what effect the degree of solvent exposure of the phenolic OH group has on the redox characteristics of tyrosine analogs. We show that the potential of the PhO•/PhOH redox pair is dominated by interactions with the OH group and that the environment around the hydrophobic part of the phenol has no significant impact. In addition, we observe that interactions between the phenolic OH group and the protein matrix can raise the phenol potential by 0.11-0.12 V relative to solution values. The α3C system is extended in Paper V to study quinone redox chemistry. Papers III and V contain protocols to generate the cofactor-containing α3C systems and descriptions of their protein properties. Paper IV describes efforts to redesign α3Y (a Trp-32 to Tyr-32 variant of α3W) to contain an interacting Tyr-32/histidine pair. The aim is to engineer and study the effects of a redox-induced proton acceptor in the Tyr-32 site." @default.
- W620671009 created "2016-06-24" @default.
- W620671009 creator A5037938392 @default.
- W620671009 date "2008-01-01" @default.
- W620671009 modified "2023-10-03" @default.
- W620671009 title "Exploring amino-acid radicals and quinone redox chemistry in model proteins" @default.
- W620671009 cites W105526937 @default.
- W620671009 cites W13155423 @default.
- W620671009 cites W1487590544 @default.
- W620671009 cites W1507516414 @default.
- W620671009 cites W1508531955 @default.
- W620671009 cites W1557027001 @default.
- W620671009 cites W1596000517 @default.
- W620671009 cites W1679806024 @default.
- W620671009 cites W1723953914 @default.
- W620671009 cites W1866116049 @default.
- W620671009 cites W1901656938 @default.
- W620671009 cites W1961323804 @default.
- W620671009 cites W1961714136 @default.
- W620671009 cites W1963515928 @default.
- W620671009 cites W1969726382 @default.
- W620671009 cites W1973861730 @default.
- W620671009 cites W1974226254 @default.
- W620671009 cites W1975967012 @default.
- W620671009 cites W1981664420 @default.
- W620671009 cites W1983435629 @default.
- W620671009 cites W1983600705 @default.
- W620671009 cites W1984893961 @default.
- W620671009 cites W1986305567 @default.
- W620671009 cites W1987768765 @default.
- W620671009 cites W1989157562 @default.
- W620671009 cites W1989744091 @default.
- W620671009 cites W1991593993 @default.
- W620671009 cites W1992152234 @default.
- W620671009 cites W1993552306 @default.
- W620671009 cites W1995022550 @default.
- W620671009 cites W1995430405 @default.
- W620671009 cites W1995679029 @default.
- W620671009 cites W1997160871 @default.
- W620671009 cites W2002551641 @default.
- W620671009 cites W2003145509 @default.
- W620671009 cites W2005034833 @default.
- W620671009 cites W2005170066 @default.
- W620671009 cites W2007606609 @default.
- W620671009 cites W2008866677 @default.
- W620671009 cites W2009198549 @default.
- W620671009 cites W2012807964 @default.
- W620671009 cites W2014059004 @default.
- W620671009 cites W2016162029 @default.
- W620671009 cites W2016891755 @default.
- W620671009 cites W2019222450 @default.
- W620671009 cites W2021872718 @default.
- W620671009 cites W2023756858 @default.
- W620671009 cites W2024610281 @default.
- W620671009 cites W2032729590 @default.
- W620671009 cites W2034570625 @default.
- W620671009 cites W2035408510 @default.
- W620671009 cites W2035564667 @default.
- W620671009 cites W2037473913 @default.
- W620671009 cites W2038917780 @default.
- W620671009 cites W2039839088 @default.
- W620671009 cites W2042397788 @default.
- W620671009 cites W2043126939 @default.
- W620671009 cites W2043175324 @default.
- W620671009 cites W2044350546 @default.
- W620671009 cites W2044557717 @default.
- W620671009 cites W2046994420 @default.
- W620671009 cites W2047167565 @default.
- W620671009 cites W2049467526 @default.
- W620671009 cites W2051248004 @default.
- W620671009 cites W2052315960 @default.
- W620671009 cites W2052918499 @default.
- W620671009 cites W2055550067 @default.
- W620671009 cites W2055843587 @default.
- W620671009 cites W2057571164 @default.
- W620671009 cites W2058182576 @default.
- W620671009 cites W2058330998 @default.
- W620671009 cites W2059918834 @default.
- W620671009 cites W2073347601 @default.
- W620671009 cites W2074047734 @default.
- W620671009 cites W2075266380 @default.
- W620671009 cites W2075708073 @default.
- W620671009 cites W2078023361 @default.
- W620671009 cites W2078362683 @default.
- W620671009 cites W2080725841 @default.
- W620671009 cites W2081143109 @default.
- W620671009 cites W2082613359 @default.
- W620671009 cites W2084086913 @default.
- W620671009 cites W2084613856 @default.
- W620671009 cites W2085972813 @default.
- W620671009 cites W2089638668 @default.
- W620671009 cites W2090295734 @default.
- W620671009 cites W2090985291 @default.
- W620671009 cites W2092019850 @default.
- W620671009 cites W2094153353 @default.
- W620671009 cites W2098918432 @default.
- W620671009 cites W2100492915 @default.
- W620671009 cites W2101231282 @default.
- W620671009 cites W2103199894 @default.
- W620671009 cites W2103854719 @default.