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- W621226015 abstract "建立肿瘤耐药细胞系是研究肿瘤细胞的重要手段之一，也是探讨肿瘤多药耐药机制及其逆转的基础。采用大剂量间歇诱导法诱导 MCF-7细胞，建立 MCF-7/Adm耐药模型；MTT 法检测耐药倍数；流式细胞术检测MCF-7细胞和MCF-7/Adm细胞中阿霉素的积累量；real-time PCR检测MCF-7/Adm细胞中 A BCG2、A BCC1、A B-CB1基因的表达。结果显示，阿霉素抑制 MCF-7和 MCF-7/Adm 细胞存活的 IC50值分别为0．535μg/mL 和173.275μg/mL ，耐药倍数为324；MCF-7细胞中的阿霉素积累量较MCF-7/Adm明显高；MCF-7/Adm细胞中mR-NA水平 A BCB1基因表达明显上调，A BCG2和 A BCC1基因表达下调。成功获得对阿霉素耐药的人乳腺癌耐药细胞株MCF-7/Adm ，为进一步研究乳腺癌多药耐药机制及其逆转提供有利工具，并且对乳腺癌化疗药物的筛选具有一定的意义。" @default.
- W621226015 created "2016-06-24" @default.
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- W621226015 date "2014-01-01" @default.
- W621226015 modified "2023-09-24" @default.
- W621226015 title "阿霉素耐药的乳腺癌MCF-7／Adm细胞系的建立及其耐药特性" @default.
- W621226015 hasPublicationYear "2014" @default.
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