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• W622272164 startingPage "54" @default.
• W622272164 abstract "In the present study, ultrasound-mediated microbubble destruction (UMMD) alone and combined with liposome technology was used as a novel nonviral technique to transfect a Nogo receptor (Nogo-R) shRNA plasmid (pNogo-R shRNA) into neural stem cells (NSCs). Using green fluorescent protein as a reporter gene, transfection efficiency of NSCs was significantly higher in the group transfected with UMMD combined with liposomes compared with that of the group transfected with UMMD or liposomes alone, and did not affect cell vitality. In addition, Nogo-R mRNA and protein expression was dramatically decreased in the UMMD combined with liposome-mediated group compared with that of other groups after 24 hours of transfection. The UMMD technique combined with liposomes is a noninvasive gene transfer method, which showed minimal effects on cell viability and effectively increased transfer of Nogo-R shRNA into NSCs." @default.
• W622272164 created "2016-06-24" @default.
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• W622272164 date "2012-01-05" @default.
• W622272164 modified "2023-10-16" @default.
• W622272164 title "Ultrasound microbubbles combined with liposome-mediated pNogo-R shRNA delivery into neural stem cells." @default.
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• W622272164 doi "https://doi.org/10.3969/j.issn.1673-5374.2012.01.009" @default.
• W622272164 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4354117" @default.
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• W622272164 hasPublicationYear "2012" @default.
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