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- W62279898 abstract "This chapter highlights the factors that must be controlled to achieve accurate diffusion assays. Diffusion assays may be put into one of three categories. One category is one-dimensional diffusion such as diffusion in test tubes or in capillary tubes. Another category is two-dimensional diffusion such as the radial diffusion from cups in the agar. The third category is three-dimensional diffusion from cylinders, beads, or discs placed on the surface of the agar layer. Linear diffusion in tubes is a practical method of assay used in Japan where it is called the superposition method. Zone size is a function of agar thickness (except for cups filled to top) and therefore, attention must be paid to achieving a uniform layer. Thin layers give larger zones than thick layers. One of the potent causes of inaccurate diffusion assays is nonuniform incubation temperature. Temperature of incubation influences both the zone size and the slope of the dose-response line. Dosing is an operation with an unavoidable inherent error. Current practice is to measure the dose accurately with an automatic pipet. The doses are put into a hole in the agar, in a cylinder, or on a disc, one at a time. Zone diameters may be measured directly by a millimeter scale, vernier caliper, Fisher-Lilly Antibiotic Zone Reader, or after projection." @default.
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- W62279898 date "1972-01-01" @default.
- W62279898 modified "2023-09-23" @default.
- W62279898 title "AN APPROACH TO ACCURATE DIFFUSION ASSAYS" @default.
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- W62279898 doi "https://doi.org/10.1016/b978-0-12-403502-7.50010-7" @default.
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