FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W62346892> ?p ?o ?g. }

• W62346892 endingPage "19704" @default.
• W62346892 startingPage "19698" @default.
• W62346892 abstract "Abstract The biochemical nature of endogenous interleukin-6 (IL-6) as it exists in human serum or plasma was investigated. Serum from a patient following bone marrow (BM) transplantation and fresh plasma samples from patients with epidermolysis bullosa or psoriasis, as well as from normal volunteers, were fractionated through G-200 columns and each of the eluted fractions assayed for IL (interleukin)-6 content using enzyme-linked immunosorbent assays (ELISAs) based on the monoclonal antibody (mAb) pairs IG61/5IL6 or 4IL6/5IL6 and in the B9 hybridoma growth factor bioassay. The IG61/5IL6 ELISA and the B9 assay detected IL-6 in BM serum almost exclusively of molecular mass approximately 20 kDa. In contrast, the 4IL6/5IL6 ELISA detected strong IL-6 immunoreactivity in complexes of size 100-150 and 400-500 kDa. IL-6 present in the 100-150- and 400-500-kDa complexes was purified by immunoaffinity chromatography through a 5IL6 mAb column. The 5IL6 mAb immunoaffinity column eluate of the respective pools from BM serum contained IL-6 at concentrations approaching 1 microgram/ml as characterized by Western blotting. Sufficient IL-6 and proteins were purified by 5IL6 mAb immunoaffinity column chromatography of the 100-150-kDa complex from 0.8 ml of BM serum to allow (i) verification of three of the polypeptides as IL-6 by amino-terminal sequencing (estimate of IL-6 in original serum sample: 5-10 micrograms/ml), (ii) identification by amino acid sequencing of the associated proteins as complement factor C3b (carboxyl-terminal of the alpha-chain), complement factor C4b (gamma-chain), C-reactive protein, and albumin, and (iii) detection of an associated polypeptide consistent with the soluble IL-6 receptor. Taken together, these data establish that IL-6 is present at unexpectedly high concentrations in human blood in novel biochemical complexes that include other plasma proteins, which in turn, can camouflage IL-6 immunoreactivity and bioactivity as measured in conventional assays." @default.
• W62346892 created "2016-06-24" @default.
• W62346892 creator A5036072136 @default.
• W62346892 creator A5063105528 @default.
• W62346892 creator A5066708474 @default.
• W62346892 creator A5069204960 @default.
• W62346892 creator A5082286842 @default.
• W62346892 creator A5089642964 @default.
• W62346892 date "1992-09-01" @default.
• W62346892 modified "2023-10-18" @default.
• W62346892 title "High levels of “complexed” interleukin-6 in human blood." @default.
• W62346892 cites W1490191338 @default.
• W62346892 cites W1493485729 @default.
• W62346892 cites W1510936315 @default.
• W62346892 cites W1576125847 @default.
• W62346892 cites W1595805439 @default.
• W62346892 cites W1596809011 @default.
• W62346892 cites W1657108383 @default.
• W62346892 cites W1740919505 @default.
• W62346892 cites W1855316965 @default.
• W62346892 cites W1971615288 @default.
• W62346892 cites W1989001888 @default.
• W62346892 cites W1999158892 @default.
• W62346892 cites W2002076761 @default.
• W62346892 cites W2002793170 @default.
• W62346892 cites W2020828026 @default.
• W62346892 cites W2029227802 @default.
• W62346892 cites W2034325406 @default.
• W62346892 cites W2036452124 @default.
• W62346892 cites W2038080163 @default.
• W62346892 cites W2038202846 @default.
• W62346892 cites W2057393115 @default.
• W62346892 cites W2086604654 @default.
• W62346892 cites W2088002482 @default.
• W62346892 cites W2094290207 @default.
• W62346892 cites W2094664478 @default.
• W62346892 cites W2094989950 @default.
• W62346892 cites W2095672355 @default.
• W62346892 cites W2100837269 @default.
• W62346892 cites W2101108802 @default.
• W62346892 cites W2107312689 @default.
• W62346892 cites W2135392289 @default.
• W62346892 cites W2155086540 @default.
• W62346892 cites W2158034263 @default.
• W62346892 cites W2160461104 @default.
• W62346892 cites W2163254761 @default.
• W62346892 cites W2165314511 @default.
• W62346892 cites W2313668099 @default.
• W62346892 cites W4245868295 @default.
• W62346892 doi "https://doi.org/10.1016/s0021-9258(18)41831-5" @default.
• W62346892 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1527089" @default.
• W62346892 hasPublicationYear "1992" @default.
• W62346892 type Work @default.
• W62346892 sameAs 62346892 @default.
• W62346892 citedByCount "118" @default.
• W62346892 countsByYear W623468922012 @default.
• W62346892 countsByYear W623468922013 @default.
• W62346892 countsByYear W623468922014 @default.
• W62346892 countsByYear W623468922015 @default.
• W62346892 countsByYear W623468922016 @default.
• W62346892 countsByYear W623468922018 @default.
• W62346892 countsByYear W623468922019 @default.
• W62346892 countsByYear W623468922020 @default.
• W62346892 countsByYear W623468922021 @default.
• W62346892 countsByYear W623468922022 @default.
• W62346892 crossrefType "journal-article" @default.
• W62346892 hasAuthorship W62346892A5036072136 @default.
• W62346892 hasAuthorship W62346892A5063105528 @default.
• W62346892 hasAuthorship W62346892A5066708474 @default.
• W62346892 hasAuthorship W62346892A5069204960 @default.
• W62346892 hasAuthorship W62346892A5082286842 @default.
• W62346892 hasAuthorship W62346892A5089642964 @default.
• W62346892 hasBestOaLocation W623468921 @default.
• W62346892 hasConcept C185592680 @default.
• W62346892 hasConcept C203014093 @default.
• W62346892 hasConcept C2778690821 @default.
• W62346892 hasConcept C71924100 @default.
• W62346892 hasConcept C74172505 @default.
• W62346892 hasConceptScore W62346892C185592680 @default.
• W62346892 hasConceptScore W62346892C203014093 @default.
• W62346892 hasConceptScore W62346892C2778690821 @default.
• W62346892 hasConceptScore W62346892C71924100 @default.
• W62346892 hasConceptScore W62346892C74172505 @default.
• W62346892 hasIssue "27" @default.
• W62346892 hasLocation W623468921 @default.
• W62346892 hasOpenAccess W62346892 @default.
• W62346892 hasPrimaryLocation W623468921 @default.
• W62346892 hasRelatedWork W1531601525 @default.
• W62346892 hasRelatedWork W2384464875 @default.
• W62346892 hasRelatedWork W2606230654 @default.
• W62346892 hasRelatedWork W2607424097 @default.
• W62346892 hasRelatedWork W2748952813 @default.
• W62346892 hasRelatedWork W2899084033 @default.
• W62346892 hasRelatedWork W2948807893 @default.
• W62346892 hasRelatedWork W3031052312 @default.
• W62346892 hasRelatedWork W4387497383 @default.
• W62346892 hasRelatedWork W2778153218 @default.
• W62346892 hasVolume "267" @default.

• next