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• W623493062 abstract "The endocannabinoid anandamide is an uncharged neuromodulatory lipid that is inactivated through its cellular uptake and subsequent catabolism. While the biosynthesis and degradation of AEA have been clarified in considerable detail, the mechanism of AEA uptake has remained elusive. There is a general consensus only on the fact that AEA movement through the plasma membrane is rapid, saturable, temperature-dependent. While many studies describe a transporter-mediated uptake of AEA via a selective “anandamide membrane transporter”, only a few papers proposed that the transport occurs by simple diffusion or endocytosis via caveolae/lipid rafts. As a matter of fact, the lack of cloning and expression of the purported transporter protein has prevented the development of molecular tools which could give definitive proof of the presence of a true transporter on the cell surface. In the same line, AEA analogs able to visualize AEA movement across the plasma membrane and its subsequent fate within the cell, are still missing.We synthesized and characterized a biotinylated analog of AEA (biotin-AEA) that has the same lipophilicity of the parent compound. We used biochemical assays and fluorescence microscopy employing b-AEA as a tool to visualize accumulation, intracellular distribution and trafficking of AEA inside the cells. We chose to modify the polar head of AEA because this structural change does not influence the kinetics of AEA uptake. Our studies led us to clarify the presence of molecular structures, the adiposomes, as a way to accumulate AEA and that could be involved in its delivery to FAAH. Using our biotinylated probe, we also identified two cytosolic proteins (albumin and Hsp70.2) as potential AEA-binding carriers which might form a delivery system to rapidly and efficiently assist the intracellular trafficking of AEA." @default.
• W623493062 created "2016-06-24" @default.
• W623493062 creator A5048608909 @default.
• W623493062 date "2010-01-27" @default.
• W623493062 modified "2023-09-27" @default.
• W623493062 title "Biotin-anandamide: a new tool to visualize anandamide inside the cells" @default.
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