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- W62460712 abstract "Bovine adrenal medullary cells were isolated in large quantity by sequential digestion of adrenal medulla with collagenase. Acetylcholine and other cholinergic agents stimulated catecholamine release and 45 Ca uptake by these isolated medullary cells. The cholinergic receptors were found to be predominantly nicotinic. The response of the isolated cells to the cholinergic drugs increased when the cells were cultured for three days. Catecholamine release was also stimulated by the divalent cation ionophores A23187 and X-537A. DB-cAMP and DB-cGMP did not stimulate catecholamine release. The synthesis of 14 C-catecholamine from 14 C-tyrosine was increased by the cholinergic agents, ouabain and excess K + in the presence of Ca ++ . DB-cAMP also stimulated the synthesis of 14 C-catecholamine, but its effect was not significantly influenced by the absence of Ca ++ . These isolated bovine adrenal medullary cells provide a useful system for studies on the regulation of catecholamine synthesis and release, which are coupled with cell membrane functions." @default.
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- W62460712 date "1979-01-01" @default.
- W62460712 modified "2023-09-25" @default.
- W62460712 title "ISOLATED BOVINE ADRENAL MEDULLARY CELLS: STUDIES ON REGULATION OF CATECHOLAMINE SYNTHESIS AND RELEASE" @default.
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- W62460712 doi "https://doi.org/10.1016/b978-1-4832-8363-0.50021-5" @default.
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