FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W626183798> ?p ?o ?g. }

• W626183798 abstract "The cAMP pathway is a universal signaling pathway regulating many cellular processes including metabolic routes, growth and differentiation. However, its effects on xenobiotic biotransformation and transport systems are poorly characterized. The effect of cAMP on expression and activity of GST and MRP2 was evaluated in Caco-2 cells, a model of intestinal epithelium. Cells incubated with the cAMP permeable analog dibutyryl cyclic AMP (db-cAMP: 1,10,100 μM) for 48 h exhibited a dose-response increase in GST class α and MRP2 protein expression. Incubation with forskolin, an activator of adenylyl cyclase, confirmed the association between intracellular cAMP and upregulation of MRP2. Consistent with increased expression of GSTα and MRP2, db-cAMP enhanced their activities, as well as cytoprotection against the common substrate 1-chloro-2,4-dinitrobenzene. Pretreatment with protein kinase A (PKA) inhibitors totally abolished upregulation of MRP2 and GSTα induced by db-cAMP. In silico analysis together with experiments consisting of treatment with db-cAMP of Caco-2 cells transfected with a reporter construct containing CRE and AP-1 sites evidenced participation of these sites in MRP2 upregulation. Further studies involving the transcription factors CREB and AP-1 (c-JUN, c-FOS and ATF2) demonstrated increased levels of total c-JUN and phosphorylation of c-JUN and ATF2 by db-cAMP, which were suppressed by a PKA inhibitor. Co-immunoprecipitation and ChIP assay studies demonstrated that db-cAMP increased c-JUN/ATF2 interaction, with further recruitment to the region of the MRP2 promoter containing CRE and AP-1 sites. We conclude that cAMP induces GSTα and MRP2 expression and activity in Caco-2 cells via the PKA pathway, thus regulating detoxification of specific xenobiotics." @default.
• W626183798 created "2016-06-24" @default.
• W626183798 creator A5005097171 @default.
• W626183798 creator A5008696413 @default.
• W626183798 creator A5018074683 @default.
• W626183798 creator A5020739105 @default.
• W626183798 creator A5035052579 @default.
• W626183798 creator A5043600382 @default.
• W626183798 creator A5053574087 @default.
• W626183798 creator A5072593544 @default.
• W626183798 creator A5075073583 @default.
• W626183798 creator A5082243994 @default.
• W626183798 date "2015-09-01" @default.
• W626183798 modified "2023-10-08" @default.
• W626183798 title "Coordinated induction of GST and MRP2 by cAMP in Caco-2 cells: Role of protein kinase A signaling pathway and toxicological relevance" @default.
• W626183798 cites W1511208409 @default.
• W626183798 cites W1534764151 @default.
• W626183798 cites W1752401393 @default.
• W626183798 cites W1904678284 @default.
• W626183798 cites W1964580600 @default.
• W626183798 cites W1982921967 @default.
• W626183798 cites W1984484754 @default.
• W626183798 cites W1985051436 @default.
• W626183798 cites W1985938141 @default.
• W626183798 cites W1994219871 @default.
• W626183798 cites W1994415200 @default.
• W626183798 cites W1994952789 @default.
• W626183798 cites W2003425522 @default.
• W626183798 cites W2006214856 @default.
• W626183798 cites W2010906767 @default.
• W626183798 cites W2015467981 @default.
• W626183798 cites W2017900217 @default.
• W626183798 cites W2021595662 @default.
• W626183798 cites W2022510326 @default.
• W626183798 cites W2022713347 @default.
• W626183798 cites W2027254996 @default.
• W626183798 cites W2028328532 @default.
• W626183798 cites W2029505449 @default.
• W626183798 cites W2036224418 @default.
• W626183798 cites W2037581530 @default.
• W626183798 cites W2042094248 @default.
• W626183798 cites W2059462768 @default.
• W626183798 cites W2061955349 @default.
• W626183798 cites W2065744227 @default.
• W626183798 cites W2068099929 @default.
• W626183798 cites W2074998509 @default.
• W626183798 cites W2076174476 @default.
• W626183798 cites W2077288372 @default.
• W626183798 cites W2093908005 @default.
• W626183798 cites W2099516877 @default.
• W626183798 cites W2108244474 @default.
• W626183798 cites W2109036715 @default.
• W626183798 cites W2115014672 @default.
• W626183798 cites W2122660076 @default.
• W626183798 cites W2125263833 @default.
• W626183798 cites W2132295115 @default.
• W626183798 cites W2140790488 @default.
• W626183798 cites W2144309665 @default.
• W626183798 cites W2147566151 @default.
• W626183798 cites W2160984493 @default.
• W626183798 cites W2224734909 @default.
• W626183798 cites W2327068644 @default.
• W626183798 cites W2328358832 @default.
• W626183798 cites W2512454832 @default.
• W626183798 cites W4362218353 @default.
• W626183798 doi "https://doi.org/10.1016/j.taap.2015.06.003" @default.
• W626183798 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26049102" @default.
• W626183798 hasPublicationYear "2015" @default.
• W626183798 type Work @default.
• W626183798 sameAs 626183798 @default.
• W626183798 citedByCount "16" @default.
• W626183798 countsByYear W6261837982016 @default.
• W626183798 countsByYear W6261837982017 @default.
• W626183798 countsByYear W6261837982018 @default.
• W626183798 countsByYear W6261837982019 @default.
• W626183798 countsByYear W6261837982020 @default.
• W626183798 countsByYear W6261837982021 @default.
• W626183798 crossrefType "journal-article" @default.
• W626183798 hasAuthorship W626183798A5005097171 @default.
• W626183798 hasAuthorship W626183798A5008696413 @default.
• W626183798 hasAuthorship W626183798A5018074683 @default.
• W626183798 hasAuthorship W626183798A5020739105 @default.
• W626183798 hasAuthorship W626183798A5035052579 @default.
• W626183798 hasAuthorship W626183798A5043600382 @default.
• W626183798 hasAuthorship W626183798A5053574087 @default.
• W626183798 hasAuthorship W626183798A5072593544 @default.
• W626183798 hasAuthorship W626183798A5075073583 @default.
• W626183798 hasAuthorship W626183798A5082243994 @default.
• W626183798 hasBestOaLocation W6261837982 @default.
• W626183798 hasConcept C104317684 @default.
• W626183798 hasConcept C115466790 @default.
• W626183798 hasConcept C11960822 @default.
• W626183798 hasConcept C127561419 @default.
• W626183798 hasConcept C149011108 @default.
• W626183798 hasConcept C153911025 @default.
• W626183798 hasConcept C1629964 @default.
• W626183798 hasConcept C170493617 @default.
• W626183798 hasConcept C185592680 @default.
• W626183798 hasConcept C2779178603 @default.
• W626183798 hasConcept C2779276759 @default.

• next