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- W626857556 abstract "Some diseases in higher plants cause formation of reactive oxygen species (ROS) which acts like mediator on tolerance to oxidative stress. Several phytotoxins produced by plant pathogen fungus induce ROS by different mechanisms which damage plant cell tissues. This paper was focused to determine juglone impact (Psedocercospora fijiensis toxin) on Superoxide dismutases antioxidant system in Fougamou (ABB) and Grande naine (AAA) by native Poliacrylamide gel electrophoresis (PAGE) 10%. Samples of proteins were collected at 2, 4, 6, 8 and 48h after juglone injection on both Musa cultivar and 65 μl of them (120 μg of total protein) were applied to gels. Gels were incubated in staining solution (25 mg.ml -1 de Nitrobluetetrazolium (NTB) and 0.1 mg.ml -1 de riboflavin) and were exposured to fluorescent light. In both cultivars it was observed changes in expression patterns of superoxide dismutases between injected and not injected plants. On Grande naine (AAA) 4 h after injection an isoform of SOD disappeared respect to control. Nevertheless on Fougamou (ABB), 2 h after injection it was activated a new isoform which was observed until 48 h. In the present work it was observed a correspondence between protein patterns expression of SOD isoforms and tolerance to oxidative stress caused by the effect of juglone on Musa spp. Key words: antioxidant enzymes, Mycosphaerella fijiensis, oxidative stress, toxins" @default.
- W626857556 created "2016-06-24" @default.
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- W626857556 date "2004-04-12" @default.
- W626857556 modified "2023-09-23" @default.
- W626857556 title "Efecto de la inyección foliar de juglone sobre el sistema antioxidante de las superóxido dismutasas en dos cultivares de Musa spp" @default.
- W626857556 hasPublicationYear "2004" @default.
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