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- W627599084 abstract "BACKGROUND: Currently, the study on the repair of articular cartilage damage is prone to constructing tissue engineered bone and cartilage composite, so as to accelerate the healing by turning cartilage-bone interface to bone-bone interface. OBJECTIVE: To investigate the feasibility of constructing tissue-engineered osteochondral composite using poly (lactide-co-glyco-lide) (PLGA) and Pluronic-F127 hydrogel as the scaffold. DESIGN, TIME AND SETTING: Cell culture was performed in the Medical Research Center, the Second Affiliated Hospital of Sun Yat-sen University (Guangzhou, Guangdong, China), and animal experiments were carried out in the Experimental Animal Center of Sun Yat-sen University (Guangzhou, Guangdong, China). MATERIALS: The chondrocyte and osteoblast obtained from the knee joint and tibia periosteum of New Zealand rabbits were mixed with Pluronic-F127 and PLGA, respectively. Then the osteoblast-PLGA complex was coated with Pluronic F-127 carrying chondrocytes to prepare the tissue-engineered osteochondral composite. METHODS: Twenty athymic mice were incised at dorsal skin and subcutaneous tissue. Tissue-engineered osteochondral composite was implanted into the left side of the incision, while pure PLGA scaffold and pure Pluronic F-127 were implanted into the right side of the incision, serving as controls. MAIN OUTCOME MEASURES: Specimens were harvested two months after implantation and subjected to gross morphology and histological analysis. Type Ⅱ collagen immunohistochemistry was used to observe the expression of type Ⅱ collagen from chondrocytes. RESULTS: Two months after implantation, bone-cartilage complex emerged, which was hill of flexibility. In the botarious part, the new bony tissue connected together or to trabs, PLGA scaffold completely dissolved; In the white part, the ripe cartslaginous tissues formed, Pluronic-F127 was absorbed completely; There was a good connection between the bone tissue and the cartilage tissue, and in some section the cartilage tissue inserted in to the bone tissue. The chondrocytes were positive for type Ⅱ collagen immunohistochemistry. CONCLUSION: Pluronic-F127 and PLGA can work as good scaffolds in tissue engineering, by which the osteochondral tissue can be formed with good connection between bone tissue and cartilage tissue." @default.
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- W627599084 date "2008-07-01" @default.
- W627599084 modified "2023-09-28" @default.
- W627599084 title "聚乳酸／聚羟乙酸共聚物和Pluronic-F127凝胶为支架复合软骨细胞构建组织工程骨软骨复合组织的界面相容性" @default.
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