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• W63001887 abstract "Research Article15 May 1995free access Cell cycle regulation of human WEE1. C.H. McGowan C.H. McGowan Department of Molecular, Scripps Research Institute, La Jolla, CA 92037, USA. Search for more papers by this author P. Russell P. Russell Department of Molecular, Scripps Research Institute, La Jolla, CA 92037, USA. Search for more papers by this author C.H. McGowan C.H. McGowan Department of Molecular, Scripps Research Institute, La Jolla, CA 92037, USA. Search for more papers by this author P. Russell P. Russell Department of Molecular, Scripps Research Institute, La Jolla, CA 92037, USA. Search for more papers by this author Author Information C.H. McGowan1 and P. Russell1 1Department of Molecular, Scripps Research Institute, La Jolla, CA 92037, USA. The EMBO Journal (1995)14:2166-2175https://doi.org/10.1002/j.1460-2075.1995.tb07210.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info WEE1 kinase negatively regulates entry into mitosis by catalyzing the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase. We report here an investigation of human WEE1. Endogenous WEE1 migrates as an approximately 94 kDa protein in SDS-PAGE, substantially larger than the 49 kDa protein encoded by the original human WEE1 cDNA clone that was truncated at the 5′-end. Antibody depletion experiments demonstrate that WEE1 accounts for most of the activity that phosphorylates CDC2 on Tyr15 in an in vitro assay of HeLa cell lysates, hence it is likely to have an important role in the mitotic control of human cells. WEE1 activity was not found to be elevated in HeLa cells arrested in S phase, suggesting that unreplicated DNA does not delay M phase by hyperactivating WEE1. WEE1 activity is strongly suppressed during M phase, suggesting that negative regulation of WEE1 could be part of the mechanism by which activation of CDC2/cyclin B kinase is promoted during the G2/M transition. M phase WEE1 is re-activated in samples prepared in the absence of protein phosphatase inhibitors, demonstrating that WEE1 is inhibited by a mechanism that requires protein phosphorylation. Previous ArticleNext Article Volume 14Issue 101 May 1995In this issue RelatedDetailsLoading ..." @default.
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• W63001887 date "1995-05-01" @default.
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• W63001887 title "Cell cycle regulation of human WEE1." @default.
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• W63001887 doi "https://doi.org/10.1002/j.1460-2075.1995.tb07210.x" @default.
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