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• W63103480 abstract "The rate equation for a tight-binding inhibitor of an enzyme-catalysed first-order reversible reaction was used to derive two integrated equations. One of them covers the situations in which competitive, uncompetitive or non-competitive inhibition occurs and the other refers to the special non-competitive case where the two inhibition constants are equal. For these equations, graphical and non-linear regression methods are proposed for distinguishing between types of inhibition and for calculating inhibition constants from progress-curve data. The application of the non-linear regression to the analysis of stimulated progress curves in the presence of a tight-binding inhibitor is also presented. The results obtained are valid for any type of ‘dead-end’-complex-forming inhibitor and can be used to characterize an unknown inhibitor on the basis of progress curves." @default.
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• W63103480 date "1990-02-01" @default.
• W63103480 modified "2023-10-18" @default.
• W63103480 title "Progress-curve equations for reversible enzyme-catalysed reactions inhibited by tight-binding inhibitors" @default.
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• W63103480 doi "https://doi.org/10.1042/bj2650647" @default.
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