FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W63267655> ?p ?o ?g. }

• W63267655 endingPage "136" @default.
• W63267655 startingPage "129" @default.
• W63267655 abstract "A radioligand immunoassay for 1,25-dihydroxycholecalciferol [1,25(OH)2D3] receptors was developed utilizing a specific, high-affinity (Kd = 1.8×10(-11 M) monoclonal antibody (9A7 gamma) obtained from suspension cultures of rat spleen X mouse myeloma hybrid SP2/0-9A7. A standard curve was established, based on the competition between 1,25(OH)2[3H]D3-receptor (18 fmol/tube) and increasing concentrations of radioinert 1,25(OH)2D3-receptor (0-240 fmol/tube) for the binding site on 9A7 gamma. Samples, prepared in identical buffer, contained 0-100 fmol of receptor/tube. After an equilibrium incubation of 1,25(OH)2[3H]D3-receptor with either standard or sample (16 h at 4 degrees C), antibody-bound receptor was immunoprecipitated with rabbit anti-(rat immunoglobulin) prelinked to Staphylococcus aureus and quantified. The assay is statistically sensitive to 2 fmol of receptor/tube, with intra- and inter-assay variations of 7 and 12% respectively. Occupied, unoccupied and denatured receptor were observed to compete equally in the assay. This quantitative technique has been successfully applied to the characterization of receptors after fractionation by sedimentation analysis and DNA-cellulose chromatography. Finally, the measurement of total receptor by this assay, in conjunction with 1,25(OH)2D3 binding assays, has revealed that rachitic, normal and 1,25(OH)2D3-injected chicks have respectively 13, 20, and 56% of receptor in the occupied form. From these results we consider that this radioligand immunoassay will be a useful tool in further research focusing on quantifying 1,25(OH)2D3 receptors in tissue and cell extracts." @default.
• W63267655 created "2016-06-24" @default.
• W63267655 creator A5059989684 @default.
• W63267655 creator A5064286100 @default.
• W63267655 creator A5082886875 @default.
• W63267655 date "1984-07-01" @default.
• W63267655 modified "2023-10-15" @default.
• W63267655 title "Development of a radioligand immunoassay for 1,25-dihydroxycholecalciferol receptors utilizing monoclonal antibody" @default.
• W63267655 cites W1489644476 @default.
• W63267655 cites W1493227275 @default.
• W63267655 cites W1500525840 @default.
• W63267655 cites W1501539795 @default.
• W63267655 cites W1517876062 @default.
• W63267655 cites W1523850365 @default.
• W63267655 cites W1528214586 @default.
• W63267655 cites W1532450327 @default.
• W63267655 cites W1582284471 @default.
• W63267655 cites W1586952476 @default.
• W63267655 cites W1603801897 @default.
• W63267655 cites W1775749144 @default.
• W63267655 cites W1798892037 @default.
• W63267655 cites W1956849066 @default.
• W63267655 cites W1964684879 @default.
• W63267655 cites W1976993792 @default.
• W63267655 cites W1981119778 @default.
• W63267655 cites W1986785326 @default.
• W63267655 cites W1999985166 @default.
• W63267655 cites W2001269969 @default.
• W63267655 cites W2003575314 @default.
• W63267655 cites W2016715827 @default.
• W63267655 cites W2019847141 @default.
• W63267655 cites W2042489312 @default.
• W63267655 cites W2046719767 @default.
• W63267655 cites W2061350052 @default.
• W63267655 cites W2071170305 @default.
• W63267655 cites W2075838211 @default.
• W63267655 cites W2082942460 @default.
• W63267655 cites W2096602324 @default.
• W63267655 cites W2122150781 @default.
• W63267655 cites W2128252687 @default.
• W63267655 cites W2128342505 @default.
• W63267655 cites W2141639058 @default.
• W63267655 cites W2154567414 @default.
• W63267655 cites W3090461134 @default.
• W63267655 doi "https://doi.org/10.1042/bj2210129" @default.
• W63267655 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1144011" @default.
• W63267655 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6087801" @default.
• W63267655 hasPublicationYear "1984" @default.
• W63267655 type Work @default.
• W63267655 sameAs 63267655 @default.
• W63267655 citedByCount "30" @default.
• W63267655 countsByYear W632676552012 @default.
• W63267655 countsByYear W632676552014 @default.
• W63267655 countsByYear W632676552016 @default.
• W63267655 countsByYear W632676552018 @default.
• W63267655 crossrefType "journal-article" @default.
• W63267655 hasAuthorship W63267655A5059989684 @default.
• W63267655 hasAuthorship W63267655A5064286100 @default.
• W63267655 hasAuthorship W63267655A5082886875 @default.
• W63267655 hasBestOaLocation W632676552 @default.
• W63267655 hasConcept C153911025 @default.
• W63267655 hasConcept C159654299 @default.
• W63267655 hasConcept C170493617 @default.
• W63267655 hasConcept C176685330 @default.
• W63267655 hasConcept C185592680 @default.
• W63267655 hasConcept C203014093 @default.
• W63267655 hasConcept C2780420688 @default.
• W63267655 hasConcept C2909005528 @default.
• W63267655 hasConcept C43617362 @default.
• W63267655 hasConcept C542903549 @default.
• W63267655 hasConcept C55493867 @default.
• W63267655 hasConcept C67847695 @default.
• W63267655 hasConcept C86803240 @default.
• W63267655 hasConceptScore W63267655C153911025 @default.
• W63267655 hasConceptScore W63267655C159654299 @default.
• W63267655 hasConceptScore W63267655C170493617 @default.
• W63267655 hasConceptScore W63267655C176685330 @default.
• W63267655 hasConceptScore W63267655C185592680 @default.
• W63267655 hasConceptScore W63267655C203014093 @default.
• W63267655 hasConceptScore W63267655C2780420688 @default.
• W63267655 hasConceptScore W63267655C2909005528 @default.
• W63267655 hasConceptScore W63267655C43617362 @default.
• W63267655 hasConceptScore W63267655C542903549 @default.
• W63267655 hasConceptScore W63267655C55493867 @default.
• W63267655 hasConceptScore W63267655C67847695 @default.
• W63267655 hasConceptScore W63267655C86803240 @default.
• W63267655 hasIssue "1" @default.
• W63267655 hasLocation W632676551 @default.
• W63267655 hasLocation W632676552 @default.
• W63267655 hasLocation W632676553 @default.
• W63267655 hasLocation W632676554 @default.
• W63267655 hasOpenAccess W63267655 @default.
• W63267655 hasPrimaryLocation W632676551 @default.
• W63267655 hasRelatedWork W1574798273 @default.
• W63267655 hasRelatedWork W1592441904 @default.
• W63267655 hasRelatedWork W1969688909 @default.
• W63267655 hasRelatedWork W1994420353 @default.
• W63267655 hasRelatedWork W2026344709 @default.

• next