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- W634428170 abstract "We studied the effect of diltiazem on bovine aortic smooth muscle cells (SMCs) in culture. SMCs were grown in Waymouth's medium supplemented with 10% fetal bovine serum for 14 days. The SMCs cultured with 10-4M diltiazem showed short-spindle or polygonal endothelial cell-like forms, keeping a cobblestone-like monolayer until the end of the 14-day culture and factor VIII related antigen (F-VIII-RAg) was proved by immunofluorescent staining. When the medium was changed into Waymouth's medium without diltiazem, the usual state of SMCs showing spindle-shaped, hills and valleys-like multilayer proliferation was recovered and then F-VIII-RAg was negative. Diltiazem (10-4M) showed a similar effect on the SMCs in primary culture by explant method. However, the effect of diltiazem on enzyme-dispersed SMCs in primary culture was slightly different. They became round, but did not show complete monolayer and tended to pile up partially. The results indicate the possibility that the effect of diltiazem on the enzyme-dispersed primary culture is slightly different from that on primary culture by explant method or subculture.The effect of diltiazem on cultured SMCs is different from that of low [Ca2+] circumstance. Proliferation of cultured SMCs was suppressed dose-dependently by adding Ca2+ chelating agent, EDTA, however the morphology was the same as that of control SMCs and F-VIII-RAg was negative. From these results, the effect of diltiazem on SMCs is not explained only by blocking Ca2+-influx into the cells. At least, diltiazem of relatively high concentration (10-4M) must have other site(s) of action, probably intracellular.Theophylline (10-3M) or dibutyryl cyclic AMP (10-3M) had similar effect on cultured SMCs to that of diltiazem. Both Ca2+ and cyclic AMP are intracellular second messengers and the interrelation of these is so complicated that it is difficult to analyse these results.Our results show the important role of intracellular Ca2+ and cyclic AMP in the transformation to oval or polygonal shape, formation of contact-inhibited monolayer, and production of F-VIII-RAg by SMCs." @default.
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- W634428170 date "1984-01-01" @default.
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- W634428170 title "The Effect of Ca<sup>2+</sup>-Antagonists on Bovine Aortic Smooth Muscle Cells in Culture (II)" @default.
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- W634428170 doi "https://doi.org/10.5551/jat1973.12.5_1123" @default.
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