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- W635438052 abstract "Absent or defective craniofacial skeletal muscle can lead to loss of function andaesthetics. Current therapies are fraught with limitations: for example, the surgicaltransfer of tissue is associated with donor site morbidity coupled with a paucity ofavailable tissue. The potential to engineer skeletal muscle tissue could circumventdisadvantages related to current techniques. Furthermore, the creation of a muscle testbedcould provide the means to investigate the response to various manipulations. Theaim of this research was to produce an in vitro human craniofacial skeletal muscle tissuesuitable as a test-bed for novel therapies involving the craniofacial region.Degradable phosphate-based glass fibre scaffolds of various configurations, combinedwith extracellular matrix (ECM) components, were seeded with human craniofacialmuscle-derived cell cultures. Myogenicity was confirmed with immunofluorescenttechniques prior to seeding. The seeded scaffolds were incubated at 37°C in ahumidified atmosphere of 5% CO2 in air for up to 21 days. Modulation contrastmicroscopy was used to analyse migration and morphology. Cell attachment andsurvival were assessed with the CyQUANT® and alamarBlue® assays, and celldifferentiation and maturation were investigated using immunofluorescence andquantitative RT-PCR.Parallel arrays of glass fibres coated with ECM components provided the correcttopology to support cell alignment and differentiation. Specifically, compared to controlscaffolds, glass fibre scaffolds promoted upregulation of developmental, fast and slowmyosin heavy chain genes. Further refinement of the system involved glass fibresembedded within collagen gels, created to mimic the architecture of native skeletalmuscle: cells within these constructs were aligned parallel to the glass fibres, and overtime, the constructs rolled along the short axis to produce a muscle ‘organoid’.Additionally, the collagen gel contracted along the long axis to reveal tufts of glassfibres analogous to tendons (mean 13.87% reduction in length). Upregulation of themyosin heavy chain genes was promoted, albeit at a later timepoint.In conclusion, degradable glass fibre-ECM scaffolds provided the correct topographicaland biological cues to aid the in vitro engineering of human craniofacial skeletal muscletissue." @default.
- W635438052 created "2016-06-24" @default.
- W635438052 creator A5046824713 @default.
- W635438052 date "2010-11-28" @default.
- W635438052 modified "2023-09-26" @default.
- W635438052 title "The in vitro engineering of craniofacial muscle constructs utilising degradable composite glass fibre-collagen scaffolds" @default.
- W635438052 hasPublicationYear "2010" @default.
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