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- W635504751 abstract "An efficient method for in vitro propagation of Solanum nigrum L has been developed. The nodal explants were cultured on MS medium supplemented with different concentrations of BAP and KIN. The two cytokinins tested, KIN was found to respond well in shoot multiplication and number of shoots from the nodal explants when compared to BAP. Large number of shoots was produced from all the concentrations of both the cytokinins. The highest frequency of 100% shoot induction was observed on MS medium supplemented with 10-15 μM KIN and BAP. The number of shoots produced on the basal medium supplemented with BAP ranged between 40 and 46 and 43 and 49 with KIN 30 days after inoculation. Excised shoots were transferred to rooting medium containing different concentrations of IBA and 2,4-D for root induction. The roots were initiated and well developed on a medium fortified with 10-15 μM of both the auxins. Of the two auxins tested, more number of roots were produced on the medium containing IBA. Maximum number of 47 roots per shoot were produced in 30 days on MS medium supplemented with 10 μM IBA. The well rooted plantlets were transplanted to the paper cup for hardening and the well established plants were transferred to the field for acclimatization." @default.
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- W635504751 date "2011-01-01" @default.
- W635504751 modified "2023-09-24" @default.
- W635504751 title "An efficient protocol for in vitro propagation of Solanum nigrum L. from nodal explants." @default.
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