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- W636143311 abstract "Omega(ω) is the smallest subunit of bacterial RNA polymerase(RNAP) core enzyme,which consists of five subunits(2α,β,β′,and ω),and is the only subunit that is not essential for the RNAP enzyme activity and cell survival.Although ω subunit is widely distributed and highly conserved in various bacterial species,the exact role of ω in bacteria is largely unknown.Genome sequencing and annotation revealed that,the genome of Xanthomonas campestris pv.campestris strain 8004(Xcc8004) contains a gene,rpoZXcc(ORF number XC0957),encoding the ω subunit of the Xcc RNAP.The present study has been performed to investigate the function of ω subunit of RNAP in Xcc,a non-polar mutant of rpoZXcc,NK0957,was constructed using plasmid pK18mob integration.Phenotype analysis showed that,the growth rate of NK0957 in the basal medium MMX and the rich medium NYG was significantly slower than that of the wild type strain Xcc8004.The slow-growth phenotype of NK0957 could be corrected by rpoZXcc in trans.These results implied that,the ω subunit plays an important role in the cell growth of Xcc." @default.
- W636143311 created "2016-06-24" @default.
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- W636143311 date "2010-01-01" @default.
- W636143311 modified "2023-09-24" @default.
- W636143311 title "Inactivation of rpoZXcc, a gene encodes the ω subunit of RNAP, results in reduced growth rate of Xanthomonas campestris pv. campestris." @default.
- W636143311 hasPublicationYear "2010" @default.
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