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- W636248974 abstract "The aim of this thesis was to verify the functionality and optimize the conditions for primers, whose products can be applied to TGGE. Primers were obtained from literature both universal primers for Eubacteria and selected specific primers for some taxonomic groups of bacteria in the Eubacteria group. Primers were tested for Alfaproteobacteria, Betaproteobacteria, Gammaproteobacteria, and Actinobacteria. Also, some reaction parameters were optimized with these primers. DNA isolated from activated sludge from wastewater treatment plants was used as the template DNA. The functionality of all primers were verified, except Alfaproteobacteria which repeatedly failed to provide the expected product together with determining DNA. As a general conclusion it can be said that reducing concentration of primers to 0,8 pmol/?l is the most proper for primer optimalization." @default.
- W636248974 created "2016-06-24" @default.
- W636248974 creator A5083199671 @default.
- W636248974 date "2011-05-18" @default.
- W636248974 modified "2023-09-23" @default.
- W636248974 title "Využití PCR pro studium mikrobiologických biodegradačních procesů" @default.
- W636248974 hasPublicationYear "2011" @default.
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