Matches in SemOpenAlex for { <https://semopenalex.org/work/W637047203> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W637047203 abstract "Major recent improvements in biotechnology have led to an accelerated production of DNA sequences. The completion of the human genome sequence, along with the genomes of more than two hundred other species, has marked the arrival of the genome era. The ultimate goal is to understand the structure and function of genomes and their genes. This thesis has focused on the computational analysis of complementary DNA (cDNA) sequences. These are copies of mRNA transcripts that correspond to the coding regions of genomes. Studying the expression patterns of genes is essential for understanding gene function. Many gene expression profiling techniques generate short sequence tags that derive from transcripts. A pilot study was performed to assess the feasibility of using the pyrosequencing platform for gene expression analysis. The sequences generated by pyrosequencing in most cases (≈ 85%) were long enough (> 18 nucleotides) to uniquely identify the corresponding transcripts through database searches. Aspects of transcript identification by short sequence tags were further investigated in a number of public databases, revealing that a tag length 16-17 nucleotides was sufficient for unique identifi- cation. Longer transcript representations are obtained from expressed sequence tag (EST) sequencing. Method development for the analysis and maintenance of large EST data sets has been performed on data from poplar, which is a tree of commercial interest to the forest biotechnology industry. In 2003 a large ESTsequencing project reached > 100 000 reads, providing a unique resource for tree biology research. ESTs have been grouped into clusters and singletons that represent potential genes. Preliminary analyses have estimated gene content in Populus to be very similar to that of model organism Arabidopsis thaliana. EST data collections provide a rich source for mining polymorphisms. A software application was developed and applied to EST data from two Populus species, and candidate single nucleotide polymorphisms (SNPs) were recorded. A study of genetic variation between the species revealed a striking similarity, with orthologous pairs being > 98% identical on the protein level. Keywords: cDNA, EST, gene expression, SNP, SAGE, polymorphism, assembly, clustering, DNA sequencing, pyrosequencing, mRNA transcript, orthology, tree biotechnology, restriction enzyme" @default.
- W637047203 created "2016-06-24" @default.
- W637047203 creator A5012563352 @default.
- W637047203 creator A5047788471 @default.
- W637047203 creator A5054656710 @default.
- W637047203 creator A5065499836 @default.
- W637047203 creator A5083621323 @default.
- W637047203 date "2004-01-01" @default.
- W637047203 modified "2023-09-27" @default.
- W637047203 title "Unravelling differences in the transcriptome of two closely related populus special" @default.
- W637047203 hasPublicationYear "2004" @default.
- W637047203 type Work @default.
- W637047203 sameAs 637047203 @default.
- W637047203 citedByCount "0" @default.
- W637047203 crossrefType "journal-article" @default.
- W637047203 hasAuthorship W637047203A5012563352 @default.
- W637047203 hasAuthorship W637047203A5047788471 @default.
- W637047203 hasAuthorship W637047203A5054656710 @default.
- W637047203 hasAuthorship W637047203A5065499836 @default.
- W637047203 hasAuthorship W637047203A5083621323 @default.
- W637047203 hasConcept C104317684 @default.
- W637047203 hasConcept C105565629 @default.
- W637047203 hasConcept C125009961 @default.
- W637047203 hasConcept C14036430 @default.
- W637047203 hasConcept C141231307 @default.
- W637047203 hasConcept C149034497 @default.
- W637047203 hasConcept C150194340 @default.
- W637047203 hasConcept C162317418 @default.
- W637047203 hasConcept C187882448 @default.
- W637047203 hasConcept C51679486 @default.
- W637047203 hasConcept C54355233 @default.
- W637047203 hasConcept C61053724 @default.
- W637047203 hasConcept C70721500 @default.
- W637047203 hasConcept C86803240 @default.
- W637047203 hasConceptScore W637047203C104317684 @default.
- W637047203 hasConceptScore W637047203C105565629 @default.
- W637047203 hasConceptScore W637047203C125009961 @default.
- W637047203 hasConceptScore W637047203C14036430 @default.
- W637047203 hasConceptScore W637047203C141231307 @default.
- W637047203 hasConceptScore W637047203C149034497 @default.
- W637047203 hasConceptScore W637047203C150194340 @default.
- W637047203 hasConceptScore W637047203C162317418 @default.
- W637047203 hasConceptScore W637047203C187882448 @default.
- W637047203 hasConceptScore W637047203C51679486 @default.
- W637047203 hasConceptScore W637047203C54355233 @default.
- W637047203 hasConceptScore W637047203C61053724 @default.
- W637047203 hasConceptScore W637047203C70721500 @default.
- W637047203 hasConceptScore W637047203C86803240 @default.
- W637047203 hasLocation W6370472031 @default.
- W637047203 hasOpenAccess W637047203 @default.
- W637047203 hasPrimaryLocation W6370472031 @default.
- W637047203 hasRelatedWork W1508257211 @default.
- W637047203 hasRelatedWork W1538009364 @default.
- W637047203 hasRelatedWork W1558096929 @default.
- W637047203 hasRelatedWork W187344178 @default.
- W637047203 hasRelatedWork W1977759371 @default.
- W637047203 hasRelatedWork W1979706471 @default.
- W637047203 hasRelatedWork W2016774432 @default.
- W637047203 hasRelatedWork W2036076628 @default.
- W637047203 hasRelatedWork W2053585411 @default.
- W637047203 hasRelatedWork W2080603995 @default.
- W637047203 hasRelatedWork W2087878206 @default.
- W637047203 hasRelatedWork W2101875329 @default.
- W637047203 hasRelatedWork W2116318839 @default.
- W637047203 hasRelatedWork W2116911053 @default.
- W637047203 hasRelatedWork W2124891933 @default.
- W637047203 hasRelatedWork W2129513180 @default.
- W637047203 hasRelatedWork W2184825473 @default.
- W637047203 hasRelatedWork W2228300370 @default.
- W637047203 hasRelatedWork W328776325 @default.
- W637047203 hasRelatedWork W994263302 @default.
- W637047203 isParatext "false" @default.
- W637047203 isRetracted "false" @default.
- W637047203 magId "637047203" @default.
- W637047203 workType "article" @default.