Matches in SemOpenAlex for { <https://semopenalex.org/work/W637607364> ?p ?o ?g. }
Showing items 1 to 65 of
65
with 100 items per page.
- W637607364 abstract "A non-clonogenic assay using the redox dye Alamar Blue and primary human tumor cells (PHTC) from patients and human tumor cell lines (HTCL) was established for rapid screening and evaluation of anticancer drugs. PHTC cultures from chronic lymphocytic leukemia (CLL) and ovarian carcinoma (Ovca) were compared with renal carcinoma (ACHN) and lymphocytic (CCRF-CEM) HTLCs in response to 63 toxic or non-toxic compounds. Many of the clinically active drugs were detected by all cell systems. However, the sensitivity pattern differed considerably between the cell types and a higher proportion of clinically inactive drugs were scored as active by the cell lines compared with the primary cultures. Ovca showed the highest ratio of clinically solid tumoractive/clinically inactive agents followed by CLL. Differential drug response in the HTCL panel representing defined types of cytotoxic drug resistance was measured using the fluorometric microculture cytotoxicity assay (FMCA). Correlation analysis of drug activity pattern of 37 drugs showed a high degree of similarity between the drugs with similar mechanism of action. Classification of drugs into four different mechanistic categoriesusing a probabilistic neural network (PNN) analysis resulted in 91% correct predictions. Further studies also indicated that differential molecular function/expression patterns in the cell line panel may be used to identify drugs interacting with specific biochemical pathways. The presence of cell lines overexpressing drug efflux mechanisms in the panel did not significantly influence the mechanistic predictions. Application of the HTCL andPHTC based test systems to a series of sarcolysin oligopeptides identified one compound, P2, which showed high antitumor activity, favourable therapeutic index, retained activity against non-proliferative cell cultures and low levels of correlation with standard drugs. Similarly, suramin and some potentially interesting analoges were also tested. Like suramin, the analoges showed little sensitivity to common resistance mechanisms. However, in PHTCs all analogues were less potent than suramin except for FCE 26644 which also showed high degree of cross reactivity to suramin. The overall results indicates that the present approach may be well suited for discovery and initial pharmacologic evaluation of novel anticancer drugs." @default.
- W637607364 created "2016-06-24" @default.
- W637607364 creator A5025665100 @default.
- W637607364 date "1999-12-01" @default.
- W637607364 modified "2023-09-26" @default.
- W637607364 title "Development and Application of New Methods for Anticancer Drug Discovery and Early Evaluation" @default.
- W637607364 hasPublicationYear "1999" @default.
- W637607364 type Work @default.
- W637607364 sameAs 637607364 @default.
- W637607364 citedByCount "0" @default.
- W637607364 crossrefType "book" @default.
- W637607364 hasAuthorship W637607364A5025665100 @default.
- W637607364 hasConcept C109316439 @default.
- W637607364 hasConcept C117262875 @default.
- W637607364 hasConcept C1491633281 @default.
- W637607364 hasConcept C185592680 @default.
- W637607364 hasConcept C202751555 @default.
- W637607364 hasConcept C2780035454 @default.
- W637607364 hasConcept C502942594 @default.
- W637607364 hasConcept C54355233 @default.
- W637607364 hasConcept C55493867 @default.
- W637607364 hasConcept C71924100 @default.
- W637607364 hasConcept C81885089 @default.
- W637607364 hasConcept C86803240 @default.
- W637607364 hasConcept C98274493 @default.
- W637607364 hasConceptScore W637607364C109316439 @default.
- W637607364 hasConceptScore W637607364C117262875 @default.
- W637607364 hasConceptScore W637607364C1491633281 @default.
- W637607364 hasConceptScore W637607364C185592680 @default.
- W637607364 hasConceptScore W637607364C202751555 @default.
- W637607364 hasConceptScore W637607364C2780035454 @default.
- W637607364 hasConceptScore W637607364C502942594 @default.
- W637607364 hasConceptScore W637607364C54355233 @default.
- W637607364 hasConceptScore W637607364C55493867 @default.
- W637607364 hasConceptScore W637607364C71924100 @default.
- W637607364 hasConceptScore W637607364C81885089 @default.
- W637607364 hasConceptScore W637607364C86803240 @default.
- W637607364 hasConceptScore W637607364C98274493 @default.
- W637607364 hasLocation W6376073641 @default.
- W637607364 hasOpenAccess W637607364 @default.
- W637607364 hasPrimaryLocation W6376073641 @default.
- W637607364 hasRelatedWork W1850521859 @default.
- W637607364 hasRelatedWork W1969285468 @default.
- W637607364 hasRelatedWork W1975209118 @default.
- W637607364 hasRelatedWork W1992992082 @default.
- W637607364 hasRelatedWork W1994051567 @default.
- W637607364 hasRelatedWork W2038601532 @default.
- W637607364 hasRelatedWork W2056337625 @default.
- W637607364 hasRelatedWork W2060510604 @default.
- W637607364 hasRelatedWork W2070812481 @default.
- W637607364 hasRelatedWork W2074015341 @default.
- W637607364 hasRelatedWork W2074509197 @default.
- W637607364 hasRelatedWork W2090032649 @default.
- W637607364 hasRelatedWork W2093237197 @default.
- W637607364 hasRelatedWork W229718744 @default.
- W637607364 hasRelatedWork W2382322867 @default.
- W637607364 hasRelatedWork W2409857913 @default.
- W637607364 hasRelatedWork W54649336 @default.
- W637607364 hasRelatedWork W574950413 @default.
- W637607364 hasRelatedWork W69689512 @default.
- W637607364 hasRelatedWork W2924073126 @default.
- W637607364 isParatext "false" @default.
- W637607364 isRetracted "false" @default.
- W637607364 magId "637607364" @default.
- W637607364 workType "book" @default.