FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W638236460> ?p ?o ?g. }

• W638236460 abstract "All organisms have developed regulated mechanisms to maintain homeostasis. At the cellular level, this normal functioning of cells is regulated by expression of regulatory genes that are required for normal cell function. Most cells in multicellular organisms are capable of altering gene expression in response to extracellular signals such as elevated temperature, ischaemia/reperfusion, inflammation, infection, cytokines, and amino acid analogues. In this thesis the effects of cellular stresses in the form of elevated temperature or simulated ischaemia have been investigated. Previous studies show that elevated temperature or simulated ischaemia can induce expression of heat shock proteins (Hsps) in order to prevent misfolding of cellular proteins. Moreover, it has been shown that the stress responsive transcription factor heat shock factor-1 (HSF-1) is phosphorylated and translocates to the nucleus to bind to heat shock elements within hsp gene promoters. In addition, HSF-1 can interact with other transcription factors such as the signal transducer and activator of transcription-1 (STAT-1), which is a latent cytoplasmic transcription factor activated in response to regulatory cytokines such as interferon gamma (IFNgamma). Preliminary data shows that elevated temperature can induce expression of Hsp90 in the STAT-1 deficient cell line (U3A) treated with IFNa (activates STAT-1 and STAT-2), but reduces the levels of Hsp90 expression in the U3A cell line treated with IFNa and IFNgammain combination. These findings suggest that there may be competition between STAT-1 homodimers and STAT-1/STAT-2 heterodimers and will require further investigation The STAT-1 transcription factor has previously been demonstrated to play a role in stress-induced apoptosis. In this study, STAT-1 is shown to be required for stress-induced apoptosis using the STAT-1 deficient U3A cell line. Cells lacking STAT-1 show reduced cell death/apoptosis in response to elevated temperature or simulated ischaemia. However, expression of STAT-1 in these cells restores sensitivity to stress-induced death. The C-terminal domain alone of STAT-1 is also able to enhance stress-induced cell death, and may be acting via a novel co-activator-type mechanism. Many protective agents have been identified that are able to reduce cell death due to ischaemic injury. Cardiotrophin-1 (CT-1), a member of the IL-6 family of cytokines, has been shown to protect rat neonatal cardiomyocytes subjected to simulated ischaemia via the p42/p44 MAPkinase and PI-3 Kinase pathways. In addition, the unrelated peptide urocortin (Ucn) also protects cardiomyocytes via the same pathway as CT-1 in response to simulated ischaemia and both CT-1 and Ucn induce Hsp expression. In this study, Ucn has been shown to be able to induce enhanced expression of CT-1 at mRNA and protein levels in response to simulated ischaemia. Moreover, the effect is mediated by activation of the CT-1 promoter and requires the transcription factor C/EBPp /NFIL-6. This finding indicates that a common pathway exists for these two protective agents with Ucn inducing CT-1 synthesis. Overall, the work performed indicates that multiple interacting pathways modulate the cellular stress response with either protective or damaging effects." @default.
• W638236460 created "2016-06-24" @default.
• W638236460 creator A5074774824 @default.
• W638236460 date "2005-01-01" @default.
• W638236460 modified "2023-09-23" @default.
• W638236460 title "Regulation of gene expression and survival in cellular stress" @default.
• W638236460 hasPublicationYear "2005" @default.
• W638236460 type Work @default.
• W638236460 sameAs 638236460 @default.
• W638236460 citedByCount "0" @default.
• W638236460 crossrefType "dissertation" @default.
• W638236460 hasAuthorship W638236460A5074774824 @default.
• W638236460 hasConcept C104317684 @default.
• W638236460 hasConcept C137984847 @default.
• W638236460 hasConcept C150194340 @default.
• W638236460 hasConcept C153911025 @default.
• W638236460 hasConcept C171958077 @default.
• W638236460 hasConcept C205260736 @default.
• W638236460 hasConcept C2776667301 @default.
• W638236460 hasConcept C2778277574 @default.
• W638236460 hasConcept C2778923194 @default.
• W638236460 hasConcept C52981337 @default.
• W638236460 hasConcept C55493867 @default.
• W638236460 hasConcept C62478195 @default.
• W638236460 hasConcept C68991219 @default.
• W638236460 hasConcept C86339819 @default.
• W638236460 hasConcept C86803240 @default.
• W638236460 hasConcept C95444343 @default.
• W638236460 hasConceptScore W638236460C104317684 @default.
• W638236460 hasConceptScore W638236460C137984847 @default.
• W638236460 hasConceptScore W638236460C150194340 @default.
• W638236460 hasConceptScore W638236460C153911025 @default.
• W638236460 hasConceptScore W638236460C171958077 @default.
• W638236460 hasConceptScore W638236460C205260736 @default.
• W638236460 hasConceptScore W638236460C2776667301 @default.
• W638236460 hasConceptScore W638236460C2778277574 @default.
• W638236460 hasConceptScore W638236460C2778923194 @default.
• W638236460 hasConceptScore W638236460C52981337 @default.
• W638236460 hasConceptScore W638236460C55493867 @default.
• W638236460 hasConceptScore W638236460C62478195 @default.
• W638236460 hasConceptScore W638236460C68991219 @default.
• W638236460 hasConceptScore W638236460C86339819 @default.
• W638236460 hasConceptScore W638236460C86803240 @default.
• W638236460 hasConceptScore W638236460C95444343 @default.
• W638236460 hasLocation W6382364601 @default.
• W638236460 hasOpenAccess W638236460 @default.
• W638236460 hasPrimaryLocation W6382364601 @default.
• W638236460 hasRelatedWork W1569171255 @default.
• W638236460 hasRelatedWork W1648663357 @default.
• W638236460 hasRelatedWork W1804323425 @default.
• W638236460 hasRelatedWork W1984791165 @default.
• W638236460 hasRelatedWork W2017055517 @default.
• W638236460 hasRelatedWork W2020290440 @default.
• W638236460 hasRelatedWork W2024184861 @default.
• W638236460 hasRelatedWork W2027507333 @default.
• W638236460 hasRelatedWork W2039095328 @default.
• W638236460 hasRelatedWork W2054838508 @default.
• W638236460 hasRelatedWork W2060480804 @default.
• W638236460 hasRelatedWork W2071960601 @default.
• W638236460 hasRelatedWork W2085628608 @default.
• W638236460 hasRelatedWork W2103618709 @default.
• W638236460 hasRelatedWork W2104843183 @default.
• W638236460 hasRelatedWork W2144870875 @default.
• W638236460 hasRelatedWork W2152276217 @default.
• W638236460 hasRelatedWork W2157504723 @default.
• W638236460 hasRelatedWork W2460716556 @default.
• W638236460 hasRelatedWork W2884219988 @default.
• W638236460 isParatext "false" @default.
• W638236460 isRetracted "false" @default.
• W638236460 magId "638236460" @default.
• W638236460 workType "dissertation" @default.