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• W63859972 abstract "This chapter discusses that progress has been largely through the widespread applications of chromatographic and centrifugation techniques for fractionation of cellular components and purification of enzymes, and, particularly in the case of microorganisms, to the ingenious application of basic concepts in molecular genetics to the organized search for enzymes suspected of being involved in such highly specific processes as replication, recombination, repair, and RNA maturation. The results of such studies have, in turn, been carried over to investigations on corresponding mammalian systems. The chapter reviews that occasional reference is made to enzymes of other vertebrates, microorganisms and plants, when necessary for elucidation of the properties or function of corresponding or related mammalian enzymes. Descriptions of the latter are limited largely to such aspects as specificity, cellular localization, and possible functional role, and supplemented, where necessary. The chapter also discusses that the classical terms “endonuclease” and “exonuclease” are supplemented by “endo-exonuclease,” the initial action of which is endonucleolytic. Classification of enzymes in this review is according to this division, which is based on properties no less fundamental than formation of products with 5'- or 3'-phosphate termini; in the latter case either directly or via 2', 3'-cyclic phosphates. In any event, neither of these systems fully reflects the properties of individual enzymes: in the case of DNases, one now has “restriction enzymes,” which recognize a particular oligonucleotide sequence, and “repair enzymes,” which recognize a defect; and among the RNases are the processing enzymes,” which also recognize a specific sequence and/or some structural feature." @default.
• W63859972 created "2016-06-24" @default.
• W63859972 creator A5009003036 @default.
• W63859972 creator A5029848580 @default.
• W63859972 date "1977-01-01" @default.
• W63859972 modified "2023-09-25" @default.
• W63859972 title "Mammalian Nucleolytic Enzymes" @default.
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