Matches in SemOpenAlex for { <https://semopenalex.org/work/W638681919> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W638681919 abstract "Antibodies and antibody fragments are essential tools in basic research, diagnostics and therapy. Conventional antibodies consist of two heavy and two light chains with both chains contributing to the antigen-binding site. In addition to these conventional antibodies, camelids (llamas, alpacas, dromedaries and camels) possess so-called heavy chain antibodies (hcAbs) that lack the light chains. The antigen binding site of these unusual antibodies is formed by one single domain only, the so called VHH domain. The VHH domain represents the smallest intact antigen binding fragment (~ 15 kDa) and is characterized by very high stability, solubility and specificity. These unique features render VHHs a promising alternative to conventional antibodies and antibody fragments with a multitude of possible applications.In the course of this thesis, we aimed to generate and characterize VHHs suitable for different applications ranging from biochemical studies to immunofluorescence assays and live cell imaging. In order to meet the diverse requirements of the intended downstream applications, a new selection method differing from traditional phage display, called native panning, was developed and established. In combination with the protein-protein interaction fluorescent two-hybrid (F2H) assay, a new process to identify antigen specific VHHs functional inside living cells was developed.We used the immune system of alpacas to generate VHH libraries against various antigens, ranging from small peptides to large proteins. The libraries were screened by phage display and antigen specific VHHs were identified by phage ELISA. We were able to identify antigen specific VHHs against different antigens which were characterized and their functionality was tested in various applications. Furthermore, we could demonstrate that the selection method influences which VHHs are identified and therefore needs to be chosen very carefully with regard to the intended biochemical and cell biological application. In summary, we developed and established an efficient and versatile process to screen and identify antigen specific VHHs suitable for different downstream applications." @default.
- W638681919 created "2016-06-24" @default.
- W638681919 creator A5009061135 @default.
- W638681919 date "2013-06-13" @default.
- W638681919 modified "2023-09-26" @default.
- W638681919 title "Generation and characterization of heavy chain antibodies derived from Camelids" @default.
- W638681919 hasPublicationYear "2013" @default.
- W638681919 type Work @default.
- W638681919 sameAs 638681919 @default.
- W638681919 citedByCount "0" @default.
- W638681919 crossrefType "journal-article" @default.
- W638681919 hasAuthorship W638681919A5009061135 @default.
- W638681919 hasConcept C104317684 @default.
- W638681919 hasConcept C108944566 @default.
- W638681919 hasConcept C124913957 @default.
- W638681919 hasConcept C147483822 @default.
- W638681919 hasConcept C151730666 @default.
- W638681919 hasConcept C15336307 @default.
- W638681919 hasConcept C153911025 @default.
- W638681919 hasConcept C159654299 @default.
- W638681919 hasConcept C167625842 @default.
- W638681919 hasConcept C185592680 @default.
- W638681919 hasConcept C186268636 @default.
- W638681919 hasConcept C195616568 @default.
- W638681919 hasConcept C203014093 @default.
- W638681919 hasConcept C2776471321 @default.
- W638681919 hasConcept C2777820669 @default.
- W638681919 hasConcept C2910693471 @default.
- W638681919 hasConcept C36394416 @default.
- W638681919 hasConcept C55493867 @default.
- W638681919 hasConcept C70721500 @default.
- W638681919 hasConcept C86803240 @default.
- W638681919 hasConceptScore W638681919C104317684 @default.
- W638681919 hasConceptScore W638681919C108944566 @default.
- W638681919 hasConceptScore W638681919C124913957 @default.
- W638681919 hasConceptScore W638681919C147483822 @default.
- W638681919 hasConceptScore W638681919C151730666 @default.
- W638681919 hasConceptScore W638681919C15336307 @default.
- W638681919 hasConceptScore W638681919C153911025 @default.
- W638681919 hasConceptScore W638681919C159654299 @default.
- W638681919 hasConceptScore W638681919C167625842 @default.
- W638681919 hasConceptScore W638681919C185592680 @default.
- W638681919 hasConceptScore W638681919C186268636 @default.
- W638681919 hasConceptScore W638681919C195616568 @default.
- W638681919 hasConceptScore W638681919C203014093 @default.
- W638681919 hasConceptScore W638681919C2776471321 @default.
- W638681919 hasConceptScore W638681919C2777820669 @default.
- W638681919 hasConceptScore W638681919C2910693471 @default.
- W638681919 hasConceptScore W638681919C36394416 @default.
- W638681919 hasConceptScore W638681919C55493867 @default.
- W638681919 hasConceptScore W638681919C70721500 @default.
- W638681919 hasConceptScore W638681919C86803240 @default.
- W638681919 hasLocation W6386819191 @default.
- W638681919 hasOpenAccess W638681919 @default.
- W638681919 hasPrimaryLocation W6386819191 @default.
- W638681919 hasRelatedWork W1667902581 @default.
- W638681919 hasRelatedWork W2035664421 @default.
- W638681919 hasRelatedWork W2097202353 @default.
- W638681919 hasRelatedWork W2144522742 @default.
- W638681919 hasRelatedWork W2184074859 @default.
- W638681919 hasRelatedWork W2399682110 @default.
- W638681919 hasRelatedWork W2470634803 @default.
- W638681919 hasRelatedWork W2477680848 @default.
- W638681919 hasRelatedWork W2520306381 @default.
- W638681919 hasRelatedWork W2736848146 @default.
- W638681919 hasRelatedWork W2885317421 @default.
- W638681919 hasRelatedWork W2911642573 @default.
- W638681919 hasRelatedWork W2952624793 @default.
- W638681919 hasRelatedWork W2969746976 @default.
- W638681919 hasRelatedWork W2981306135 @default.
- W638681919 hasRelatedWork W2987031884 @default.
- W638681919 hasRelatedWork W3152286598 @default.
- W638681919 hasRelatedWork W3171070535 @default.
- W638681919 hasRelatedWork W3193228342 @default.
- W638681919 hasRelatedWork W234208090 @default.
- W638681919 isParatext "false" @default.
- W638681919 isRetracted "false" @default.
- W638681919 magId "638681919" @default.
- W638681919 workType "article" @default.