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• W639057259 abstract "AMP-activated protein kinase (AMPK) serves as an energy-sensing protein that is activated by a variety of metabolic stresses. Recent studies suggest that AMPK is also regulated by hormones and by nutrients such as glucose and fatty acids. In skeletal muscle it was previously shown that AMPK activity was decreased with increasing glucose concentration. In the present study both the activity and the Threonine-172 phosphorylation of AMPK in incubated rat ventricular cardiac myocytes were found to be decreased by increasing glucose in the presence and absence of palmitate. Glucose also caused a decrease in the AMPK-driven phosphorylation of acetyl-CoA carboxylase. Measurements of the myocyte contents of ATP, ADP, AMP and glycogen showed that the effect of glucose on AMPK activity could not be secondary to changes in the levels of these metabolites. The decrease in AMPK activity with glucose was additive to and distinct from the effect of insulin which is mediated through protein kinase B (PKB). Increasing glucose concentration had no effect on the phosphorylation of Threonine-308 and Serine-473 in PKB. AICAR, a pharmacological activator of AMPK, had no effect on the ability of glucose to inactivate AMPK. The myocyte content of the pentose phosphate pathway (PPP) metabolite xylulose 5-phosphate (Xu5P), a known allosteric activator of PP2A, was increased with increasing glucose concentration such that AMPK activity was inversely related to Xu5P content. The glucose 6-phosphate dehydrogenase inhibitor diehydroepiandeosterone (DHA) and thiamine, the precursor of the coenzyme for transketolase, both increased AMPK activity whereas the NADPH oxidant phenazine methosulphate (PMS) decreased AMPK activity. DHA and PMS respectively decreased and increased flux through the PPP. The findings suggest that inactivation of AMPK by glucose may be mediated by the activity of the PPP which sets the level of Xu5P. Two other findings were also made during the course of this project. First, the activity of phosphofructokinase-2 (PFK-2) in the perfused heart was previously shown to be activated through phosphorylation by AMPK. It was therefore expected that increasing glucose concentration would decrease myocyte PFK-2 activity. However PFK-2 activity was found to be increased by glucose. Second, the phosphorylation of Threonine-308 in PKB in response to insulin was appreciably increased in the presence of AICAR suggesting that there may be crosstalk between AMPK and the insulin signalling pathway at the level of PKB." @default.
• W639057259 created "2016-06-24" @default.
• W639057259 creator A5005571116 @default.
• W639057259 date "2009-11-01" @default.
• W639057259 modified "2023-09-27" @default.
• W639057259 title "The effect of glucose on cardiac AMP-activated protein kinase" @default.
• W639057259 cites W12195305 @default.
• W639057259 cites W125579703 @default.
• W639057259 cites W134523744 @default.
• W639057259 cites W142666131 @default.
• W639057259 cites W1486421932 @default.
• W639057259 cites W1491506353 @default.
• W639057259 cites W1497671643 @default.
• W639057259 cites W1497976031 @default.
• W639057259 cites W1503255196 @default.
• W639057259 cites W1515861746 @default.
• W639057259 cites W1517759896 @default.
• W639057259 cites W1520375709 @default.
• W639057259 cites W1520751097 @default.
• W639057259 cites W1529367350 @default.
• W639057259 cites W1531062343 @default.
• W639057259 cites W1536584478 @default.
• W639057259 cites W1553733060 @default.
• W639057259 cites W155391389 @default.
• W639057259 cites W1554472057 @default.
• W639057259 cites W1556440714 @default.
• W639057259 cites W1565549817 @default.
• W639057259 cites W1572887479 @default.
• W639057259 cites W1578325385 @default.
• W639057259 cites W1583686565 @default.
• W639057259 cites W1585558973 @default.
• W639057259 cites W1592200608 @default.
• W639057259 cites W1595730385 @default.
• W639057259 cites W1601097124 @default.
• W639057259 cites W1641717686 @default.
• W639057259 cites W1647150274 @default.
• W639057259 cites W1754330898 @default.
• W639057259 cites W1763983382 @default.
• W639057259 cites W1782753704 @default.
• W639057259 cites W1802422726 @default.
• W639057259 cites W1804208390 @default.
• W639057259 cites W1810417380 @default.
• W639057259 cites W1820085648 @default.
• W639057259 cites W1821324646 @default.
• W639057259 cites W1827521892 @default.
• W639057259 cites W1833083308 @default.
• W639057259 cites W1838774522 @default.
• W639057259 cites W1845241518 @default.
• W639057259 cites W1855218428 @default.
• W639057259 cites W1871669466 @default.
• W639057259 cites W1876043561 @default.
• W639057259 cites W1887746592 @default.
• W639057259 cites W1902325539 @default.
• W639057259 cites W1908662736 @default.
• W639057259 cites W1935661054 @default.
• W639057259 cites W1951790589 @default.
• W639057259 cites W1953743184 @default.
• W639057259 cites W195721858 @default.
• W639057259 cites W1958525759 @default.
• W639057259 cites W196037030 @default.
• W639057259 cites W1964122705 @default.
• W639057259 cites W1964635345 @default.
• W639057259 cites W1966761605 @default.
• W639057259 cites W1967193277 @default.
• W639057259 cites W1970020574 @default.
• W639057259 cites W1970173886 @default.
• W639057259 cites W1971508867 @default.
• W639057259 cites W1971960342 @default.
• W639057259 cites W1972126761 @default.
• W639057259 cites W1972287981 @default.
• W639057259 cites W1972356683 @default.
• W639057259 cites W1972762474 @default.
• W639057259 cites W1972816816 @default.
• W639057259 cites W1973395730 @default.
• W639057259 cites W1973488815 @default.
• W639057259 cites W1973816983 @default.
• W639057259 cites W1974780945 @default.
• W639057259 cites W1975721027 @default.
• W639057259 cites W1976215363 @default.
• W639057259 cites W1976389048 @default.
• W639057259 cites W1976394389 @default.
• W639057259 cites W1977238230 @default.
• W639057259 cites W1977482668 @default.
• W639057259 cites W1977967386 @default.
• W639057259 cites W1978489370 @default.
• W639057259 cites W1978679812 @default.
• W639057259 cites W1978716173 @default.
• W639057259 cites W1978717617 @default.
• W639057259 cites W1978763812 @default.
• W639057259 cites W1978885288 @default.
• W639057259 cites W1979188811 @default.
• W639057259 cites W1980573776 @default.
• W639057259 cites W1980895281 @default.
• W639057259 cites W1981576729 @default.
• W639057259 cites W1982126966 @default.
• W639057259 cites W1982370712 @default.
• W639057259 cites W1983445904 @default.
• W639057259 cites W1983834588 @default.
• W639057259 cites W1983877797 @default.
• W639057259 cites W1984413737 @default.

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